Pitfalls in DNA extraction from ancient bones found in Brazilian shell-mounds

Authors

  • Daniela Gaeta Arruda Universidade de São Paulo. Faculdade de Medicina. Departamento de Psiquiatria
  • Mario Hideo Hirata Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Toxicológicas
  • Orlando Cesar de Oliveira Barretto Universidade de São Paulo. Faculdade de Medicina. Departamento de Psiquiatria

DOI:

https://doi.org/10.11606/issn.2448-1750.revmae.2007.89814

Keywords:

Shell mounds, Ancient bones, DNA extraction of ancient bones, PCR of ancient DNA

Abstract

The authors report their difficulties in DNA extraction from ancient bones found in shell-mounds of Sta. Catarina Island, SC, Brazil. The presence of humic acid, fulvic acid, free oxidant radicals and the hydrolytic effect of wet environment degrade the DNA to 20 to 30 base pairs, what jeopardizes any further utilization. The humic acid and the fulvic acid chelate magnesium ion necessary to Taq-polymerase activity, inhibiting the polymerase chain reaction (PCR). In this work the most efficient used protocols for DNA extraction, and the several PCR assay conditions are discussed.

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Published

2007-12-03

Issue

No. 17 (2007)

Section

Articles

License

Copyright (c) 2007 Daniela Gaeta Arruda, Mario Hideo Hirata, Orlando Cesar de Oliveira Barretto

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

ARRUDA, Daniela Gaeta; HIRATA, Mario Hideo; BARRETTO, Orlando Cesar de Oliveira. Pitfalls in DNA extraction from ancient bones found in Brazilian shell-mounds. Revista do Museu de Arqueologia e Etnologia, São Paulo, Brasil, n. 17, p. 461–467, 2007. DOI: 10.11606/issn.2448-1750.revmae.2007.89814. Disponível em: https://journals.usp.br/revmae/article/view/89814.. Acesso em: 19 may. 2024.