Relationship analysis of Yersinia pestis strains isolated during the epidemic focus of Araripe, Pernambuco, Brazil, by MLVA

Authors

  • Morse Edson Pessoa Junior Universidade Federal de Pernambuco. Departamento de Genética
  • Gerlane Tavares de Souza Centro de Pesquisas Aggeu Magalhães/Fiocruz. Departamento de Microbiologia
  • Silvana Santos Centro de Pesquisas Aggeu Magalhães/Fiocruz. Departamento de Microbiologia
  • Tereza Cristina Leal Balbino Centro de Pesquisas Aggeu Magalhães/Fiocruz. Departamento de Microbiologia
  • Nilma Cintra Leal Centro de Pesquisas Aggeu Magalhães/Fiocruz. Departamento de Microbiologia
  • Maria Betânia Melo de Oliveira Universidade Federal de Pernambuco. Departamento de Bioquímica
  • Alzira Maria Paiva de Almeida Centro de Pesquisas Aggeu Magalhães/Fiocruz. Departamento de Microbiologia

DOI:

https://doi.org/10.7594/revbio.11.02.02

Keywords:

MLVA, plague, VNTR.

Abstract

A set of twenty strains of Yersinia pestis isolated during the investigation of an outbreak in a focus of the Araripe, municipality of Exu, state of Pernambuco, Brazil, was analyzed for six VNTR (variable number of tandem repeats) loci. The strains, conserved in the bacterioteca SRP (bacterial collection of the Reference Service in Plague), were reactivated and the identification confirmed by bacteriological bacteriophage susceptibility to anti-plague. Changes in the genome were screened for the presence or absence of virulence genes in plasmids prototypical Y. pestis and High-Pathogenicity Island (HPI) of yersinias by multiplex-PCR. The strains proved to be genetically related by MLVA (analysis of multiple loci of variable number of tandem repeats), which reflects the epidemiological relationship of these isolates.

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Published

2018-04-23

Issue

Vol. 11 No. 2 (2014)

Section

Artigo

License

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How to Cite

Pessoa Junior, M. E., Souza, G. T. de, Santos, S., Balbino, T. C. L., Leal, N. C., Oliveira, M. B. M. de, & Almeida, A. M. P. de. (2018). Relationship analysis of Yersinia pestis strains isolated during the epidemic focus of Araripe, Pernambuco, Brazil, by MLVA. Revista Da Biologia, 11(2), 7-13. https://doi.org/10.7594/revbio.11.02.02