Determination of sulfate in algal polysaccharide samples: a step-by-step protocol using microplate reader
DOI:
https://doi.org/10.1590/2675-2824069.21-010pbtKeywords:
Sulfate, Polysaccharides, Step-by-step protocol, Microplate, SeaweedAbstract
Algal polysaccharides exhibit a wide range of biological activities and potential applications. Many of these bioactivities
correlate positively with the presence of sulfate groups on the polysaccharides. The most common method used for
sulfate quantification in algal samples is the turbidimetric method using the barium chloride-gelatin reagent. However,
the original procedure is difficult to adapt for routine analysis since it is laborious and time-consuming. An optimized
method was established using 96-well microplates, with the advantage of reducing waste and discrimination between
organic and inorganic sulfates. This proposed method produced the same accuracy as the original.
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