Effect of polyaromatic hydrocarbons on cellular cytochrome P450 1A induction
DOI:
https://doi.org/10.1590/2675-2824069.21026mgKeywords:
Aromatic hydrocarbons, Cytochrome P450, Siganus rivulatus, Antioxidants, Western blottingAbstract
The detection of cytochrome P450 1A in S. rivulatus liver and gills was used as a biomarker for the exposure to polyaromatic
hydrocarbons (PAHs) as organic pollutants along the Bay of Alexandria, Egypt. PAHs were determined in both sediment
and S. rivulatus tissues in Abu Qir Bay, Alexandria as a test area and the Matrouh coast as a control area. High levels of PAHs
in Abu Qir Bay area and ratios of specific PAH compounds indicate the petrogenic origin of hydrocarbons. Both antioxidant
activity and oxidative stress markers increased significantly. The protein expression of P450 1A in the liver and gill of S.
rivulatus was markedly induced. Moreover, the toxicity in fish organs was supported by histopathological observations.
These data suggest correlation between bioaccumulated PAH and P450 1A induction and provide strong evidence of the
importance of P450 1A as a biomarker of the exposure of S. rivulatus to xenobiotics
References
ABDALLAH, M. A. M. 2017. Bioaccumulation of hydrocarbons in
freshwater fish species cultured in a shallow Coastal Lagoon,
Egypt. Earth Systems and Environment, 1(1), 1-7.
AEBI, H. 1984. Catalase in vitro. Methods in Enzymology, 105,
-126.
AN, R., LI, V., NIU, Z. & YU, H. 2008. Responses of antioxidant
enzymes in catfish exposed to liquid crystals from E-waste.
International Journal of Environmental Research and Public
Health, 5(2), 99-103.
BACKLUND, M. & INGELMAN-SUNDBERG, M. 2005. Regulation
of aryl hydrocarbon receptor signal transduction by protein
tyrosine kinases. Cellular Signalling, 17(1), 39-48.
BEUTLER, E., DURON, O. & KELLY, B. M. 1963. Improved method
for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61, 882-888.
BOUCHET, V. M., ALVE, E., RYGG, B. & TELFORD, R. J. 2012. Benthic
foraminifera provide a promising tool for ecological quality assessment of marine waters. Ecological Indicators, 23, 66-75.
BUEGE, J. A. & AUST, S. D. 1978. Microsomal lipid peroxidation.
Methods in Enzymology, 52, 302-310.
CHIARO, C. R., PATEL, R. D., MARCUS, C. B. & PERDEW, G. H. 2007.
Evidence for an aryl hydrocarbon receptor-mediated cytochrome p450 autoregulatory pathway. Molecular Pharmacology, 72(5), 1369-1379.
DOUMAS, B. T., WASTON, W. A. & BIGGS, H. G. 1997. Albumin
standard and the measurement of serum albumin with bromocresol green. Clinica Chimica Acta, 258(1), 21-30.
GOKSØYR, A. & FÖRLIN, L. 1992. The cytochrome P-450 system
in fish, aquatic toxicology and environmental monitoring.
Aquatic Toxicology, 22(4), 287-311.
HARTL, M. G., KILEMADE, M., SHEEHAN, D., MOTHERSILL, C.,
O’HALLORAN, J., O’BRIEN, N. M. & VAN PELT, F. N. 2007. Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L. Marine Environmental Research, 64(2), 191-208.
Hydrocarbons and P4501A induction
Ocean and Coastal Research 2021, v69:e21026 8
Gaber et al.
HONDA, M. & SUZUKI, N. 2020. Toxicities of polycyclic aromatic
hydrocarbons for aquatic animals. International Journal of
Environmental Research and Public Health, 17(4), 1363.
IBOR, O. R., ADEOGUN, A. O., CHUKWUKA, A. V. & ARUKWE, A.
Gross pathology, physiological and toxicological responses in relation to metals and persistent organic pollutants (POPs) burden in tilapia species from Ogun River, Nigeria. Marine Environmental Research, 129, 245-257.
IKUE, G. S., MONANU, M. O. & ONUAH, C. L. 2016. Bioaccumulation of polycyclic aromatic hydrocarbons in tissues (gills
and muscles) of (catfish) Chrysichthys nigrodidatatus from
crude oil polluted water of Ogoniland, river state, Nigeria.
Journal of Applied Life Sciences International, 6(3), 1-6.
JANGAARD, P. M., BROCKERHOFF, H., BURGHER, R. D. &
HOYLE, R. J. 1967. Seasonal changes in general condition
and lipid content of cod from inshore waters. Journal of
the Fisheries Board of Canada, 24(3), 607-612.
JUMA, R. R., SALUM, N. S., TAIROVA, Z., STRAND, J., BAKARI,
S. S. & SHEIKH, M. A. (2018). Potential of Periophthalmus
sobrinus and Siganus sutor as bioindicator fish species
for PAH pollution in tropical waters. Regional Studies in
Marine Science, 18, 170-176.
KHAN, S. G., KATIYAR, S. K., AGARWAL, R. & MUKHTAR, H.
Enhancement of antioxidant and phase II enzymes
by oral feeding of green tea polyphenols in drinking water to SKH-1 hairless mice: possible role in cancer chemoprevention. Cancer Research, 52(14), 4050-4052.
LARANJEIRO, F., SÁNCHEZ-MARÍN, P., GALANTE-OLIVEIRA, S.
& BARROSO, C. 2015. Tributyltin pollution biomonitoring under the Water Framework Directive: proposal of a
multi-species tool to assess the ecological quality status
of EU water bodies. Ecological Indicators, 57, 525-535.
LI, Y., WANG, C., ZOU, X., FENG, Z., YAO, Y., WANG, T. & ZHANG,
C. 2019. Occurrence of polycyclic aromatic hydrocarbons (PAHs) in coral reef fish from the South China Sea.
Marine Pollution Bulletin, 139, 339-345.
LÜCHMANN, K. H., DAFRE, A. L., TREVISAN, R., CRAFT, J. A.,
MENG, X., MATTOS, J. J., ZACCHI, F. L., DORRINGTON, T. S.,
SCHOROEDER, D. C. & BAINY, A. C. 2014. A light in the darkness: new biotransformation genes, antioxidant parameters and tissue-specific responses in oysters exposed to
phenanthrene. Aquatic Toxicology, 152, 324-334.
LUSHCHAK, V. I. 2016. Contaminant-induced oxidative stress
in fish: a mechanistic approach. Fish Physiology and Biochemistry, 42(2), 711-747.
MACHALA, M., DUSEK, L., HILSCHEROVA, K., KUBINOVA, R.,
JURAJDA, P., NECA, J., ULRICH, R., GELNAR, M., STUDNICKOVÁ, Z. & HOLOUBEK, I. 2001. Determination and
multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater
fish Leuciscus cephalus L. Environmental Toxicology and
Chemistry, 20(5), 1141-1148.
MYERS, M., ANULACION, B., FRENCH, B., HOM, T., REICHERT,
W., HUFNAGLE, L. & COLLIER, T. 2000. Biomarker and
histopathologic responses in flatfish following site remediation in Eagle Harbor, WA. Marine Environmental
Research, 50(1-5), 435-436.
PAMPANIN, D. M., BROOKS, S. J., GRØSVIK, B. E., LE GOFF, J.,
MEIER, S. & SYDNES, M. O. 2017. DNA adducts in marine
fish as biological marker of genotoxicity in environmental monitoring: the way forward. Marine Environmental
Research, 125, 49-62.
PAMPANIN, D. M., LE GOFF, J., SKOGLAND, K., MARCUCCI, C. R.,
ØYSÆD, K. B., LORENTZEN, M., JORGENSEN, K. B. & SYDNES, M.
O. 2016. Biological effects of polycyclic aromatic hydrocarbons
(PAH) and their first metabolic products in in vivo exposed
Atlantic cod (Gadus morhua). Journal of Toxicology and Environmental Health, 79(13-15), 633-646.
PARENTE, T. E., OLIVEIRA, A. C. & PAUMGARTTEN, F. J. 2008.
Induced cytochrome P450 1A activity in cichlid fishes
from Guandu River and Jacarepaguá Lake, Rio de Janeiro, Brazil. Environmental Pollution, 152(1), 233-238.
PIRSAHEB, M., IRANDOST, M., ASADI, F., FAKHRI, Y. & ASADI, A. 2018.
Evaluation of polycyclic aromatic hydrocarbons (PAHs) in fish: a
review and meta-analysis. Toxin Reviews, 39(5), 1-9.
POLAND, A. & KNUTSON, J. C. 1982. 2, 3, 7, 8-Tetrachlorodibenzo-pdioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity. Annual Review of Pharmacology and Toxicology, 22, 517-554.
REYNOLDS, E. S. 1963. The use of lead citrate at high pH as
an electron-opaque stain in electron microscopy. Journal of Cell Biology, 17(1), 208-212.
SANTOS, C., OLIVEIRA, M. T., DE SYLLOS CÓLUS, I. M., SOFIA, S. H. &
MARTINEZ, C. B. R. 2018. Expression of cyp1a induced by benzo
(A) pyrene and related biochemical and genotoxic biomarkers
in the neotropical freshwater fish Prochilodus lineatus. Environmental Toxicology and Pharmacology, 61, 30-37.
SARKER, S., VASHISTHA, D., SARKER, M. S. & SARKAR, A. 2018.
DNA damage in marine rock oyster (Saccostrea cucullata) exposed to environmentally available PAHs and
heavy metals along the Arabian Sea coast. Ecotoxicology
and Environmental Safety, 151, 132-143.
SHAW, J. P., DONDERO, F., MOORE, M. N., NEGRI, A., DAGNINO, A.,
READMAN, J. W., LOWE, D. R., FRICKERS, P. E., BEESLEY, A., THAIN,
J. E. & VIARENGO, A. 2011. Integration of biochemical, histochemical and toxicogenomic indices for the assessment of
health status of mussels from the Tamar Estuary, UK. Marine
Environmental Research, 72(1-2), 13-24.
STEGEMAN, J. J. & HAHN, M. E. 1994. Biochemistry and molecular biology of monooxygenases: current perspectives on forms, functions, and regulation of cytochrome
P450 in aquatic species. Aquatic Toxicology, 87, 206.
TOWBIN, H., STAEHELIN, T. & GORDON, J. 1979. Electrophoretic
transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings
of the National Academy of Sciences U S A, 76(9), 4350-4354.
VERMA, N., PINK, M., RETTENMEIER, A. W. & SCHMITZ‐SPANKE, S. 2012. Review on proteomic analyses of benzo [a]
pyrene toxicity. Proteomics, 12(11), 1731-1755.
WANG, W., WANG, J., NIE, H., FAN, R. & HUANG, Y. 2020. Occurrence, trophic magnification and potential risk of
short-chain chlorinated paraffins in coral reef fish from
the Nansha Islands, South China Sea. Science of the Total
Environment, 739, 140084.
Hydrocarbons and P4501A induction
Ocean and Coastal Research 2021, v69:e21026 9
Gaber et al.
YUSUF, K. A., EZECHUKWU, L. N., FAKOYA, K. A., AKINTOLA,
S. L., AGBOOLA, J. I. & OMOLEYE, T. O. 2015. Influence of
fish smoking methods on polycyclic aromatic hydrocarbons content and possible risks to human health. African Journal of Food Science, 9(3), 126-135.
ZHOU, H., WU, H., LIAO, C., DIAO, X., ZHEN, J., CHEN, L. & XUE,
Q. 2010. Toxicology mechanism of the persistent organic
pollutants (POPs) in fish through AhR pathway. Toxicology Mechanisms and Methods, 20(6), 279-286.
