Novel approach to soft tissue regeneration

in vitro study of compound hyaluronic acid and horizontal platelet-rich fibrin combination

Authors

  • Yun Qiu Wuhan University, School & Hospital of Stomatology, Hubei Key Laboratory of Stomatology, State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Wuhan, China https://orcid.org/0000-0002-0333-2508
  • Kailun Shen Stomatological Hospital of Xiamen Medical School, Xiamen, China. University of Wuhan, School and Hospital of Stomatology, Department of Dental Implantology, Wuhan, China
  • Hongjiang Wei Wuhan University, School & Hospital of Stomatology, Hubei Key Laboratory of Stomatology, State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Wuhan, China
  • Yufeng Zhang Wuhan University, School & Hospital of Stomatology, Hubei Key Laboratory of Stomatology, State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Wuhan, China. SCImago image Wuhan University, School of Medicine, Medical Research Institute, Wuhan, China.
  • Yulan Wang Wuhan University, School & Hospital of Stomatology, Hubei Key Laboratory of Stomatology, State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Wuhan, China. SCImago image Wuhan University, School of Medicine, Medical Research Institute, Wuhan, China
  • Yi Bai Wuhan University, School of Medicine, Medical Research Institute, Wuhan, China

DOI:

https://doi.org/10.1590/1678-7757-2023-0294

Keywords:

Soft tissue regeneration, Hyaluronic acid, Horizontal platelet-rich fibrin, Gingival fibroblast

Abstract

Objective: This study aims to develop a compound biomaterial to achieve effective soft tissue regeneration. Methodology: Compound hyaluronic acid (CHA) and liquid horizontal-platelet-rich fibrin (H-PRF) were mixed at a ratio of 1:1 to form a CHA-PRF gel. Human gingival fibroblasts (HGFs) were used in this study. The effect of CHA, H-PRF, and the CHA-PRF gel on cell viability was evaluated by CCK-8 assays. Then, the effect of CHA, H-PRF, and the CHA-PRF gel on collagen formation and deposition was evaluated by qRT‒PCR and immunofluorescence analysis. Finally, qRT‒PCR, immunofluorescence analysis, Transwell assays, and scratch wound-healing assays were performed to determine how CHA, H-PRF, and the CHA-PRF gel affect the migration of HGFs. Results: The combination of CHA and H-PRF shortened the coagulation time of liquid H-PRF. Compared to the pure CHA and H-PRF group, the CHA-PRF group exhibited the highest cell proliferation at all time points, as shown by the CCK-8 assay. Col1a and FAK were expressed at the highest levels in the CHA-PRF group, as shown by qRT‒PCR. CHA and PRF could stimulate collagen formation and HGF migration, as observed by fluorescence microscopy analysis of COL1 and F-actin and Transwell and scratch healing assays. Conclusion: The CHA-PRF group exhibited greater potential to promote soft tissue regeneration by inducing cell proliferation, collagen synthesis, and migration in HGFs than the pure CHA or H-PRF group. CHA-PRF can serve as a great candidate for use alone or in combination with autografts in periodontal or peri-implant soft tissue regeneration.

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Published

2024-05-10 — Updated on 2024-05-10

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How to Cite

Qiu, Y., Shen, K. ., Wei, H., Zhang, Y. ., Wang, Y., & Bai, Y. (2024). Novel approach to soft tissue regeneration: in vitro study of compound hyaluronic acid and horizontal platelet-rich fibrin combination. Journal of Applied Oral Science, 32, e20230294. https://doi.org/10.1590/1678-7757-2023-0294