Bone microdamage evaluation
a supplementary tool to provide three-dimensional visualization
DOI:
https://doi.org/10.11606/issn.2357-8041.clrd.2020.170117Keywords:
Bone, Microdamage, Microcrack, Fracture, Three-Dimensional Imaging, Computer-Generated 3D ImagingAbstract
Objectives: This technical report demonstrates the use of a 3D visualization tool to evaluate bone microdamage in histological sections. Methods: Histological sections obtained from non-demineralized bone fragments stained with Calcein and Xylenol Orange and embedded in methyl methacrylate were used. Sections containing microdamages (microcracks, diffuse damage and/or microfractures) were assessed to create a 3D image with the use of polarization and phase-contrast microscopies by light optical and confocal microscopes, respectively. The 3D images were created with the use of a plugin named “Interactive 3D Surface Plot” for ImageJ. Results: Microdamage morphology was better evaluated by means of the 3D image obtained, visualized in both plots of original colors and Spectrum LUT multicolor. The different colors in the Spectrum LUT highlighted the damage and improved visualization. Conclusions: The use of 3D visualization to supplement classic bone microdamage evaluation improves the assessment of damage morphology and enhances their analysis by changing the perspective in different views.
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Dominguez VM, Agnew AM. Microdamage as a bone quality component: practical guidelines for the two-dimensional analysis of linear microcracks in human cortical bone. JBMR Plus. 2019 Jul 17;3(6):e10203. doi: 10.1002/jbm4.10203
Chapurlat RD, Delmas PD. Bone microdamage: a clinical perspective. Osteoporos Int. 2009 Aug;20(8):1299-308. doi: https://doi.org/10.1007/s00198-009-0899-9
Dooley C, Tisbo P, Lee TC, Taylor D. Rupture of osteocyte processes across microcracks: the effect of crack length and stress. Biomech Model Mechanobiol. 2012 Jul;11(6):759-66. doi: https://doi.org/10.1007/s10237-011-0349-4
Green JO, Nagaraja S, Diab T, Vidakovic B, Guldberg RE. Age-related changes in human trabecular bone: relationship between microstructural stress and strain and damage morphology. J Biomech. 2011 Aug 11;44(12):2279-85. doi: https://doi.org/10.1016/j.jbiomech.2011.05.034
Vashishth D, Tanner KE, Bonfield W. Contribution, development and morphology of microcracking in cortical bone during crack propagation. J Biomech. 2000 Sep;33(9):1169-74. doi: 10.1016/s0021-9290(00)00010-5
Lee TC, Staines A, Taylor D. Bone adaptation to load: Microdamage as a stimulus for bone remodelling. J Anat. 2002 Dec;201(6):437-46. doi: https://doi.org/10.1046/j.1469-7580.2002.00123.x
Rabelo GD, Portero-Muzy N, Gineyts E, Roux JP, Chapurlat R, Chavassieux P. Spatial distribution of microcracks in osteoarthritic femoral neck: influence of osteophytes on microcrack formation. Calcif Tissue Int. 2018 Dec;103(6):617-24. doi: https://doi.org/10.1007/s00223-018-0456-7
Portero-Muzy NR, Chavassieux PM, Arlot ME, Chapurlat RD. Staining procedure for the detection of microcracks: application to ewe bone. Bone. 2011 Oct;49(4):917-9. doi: https://doi.org/10.1016/j.bone.2011.07.001
Farlay D, Boivin G. Bone mineral quality [Internet]. IntechOpen. 2012 [cited 2019 May 19]. doi: https://doi.org/10.13140/2.1.1530.4644
O’Brien FJ, Taylor D, Lee TC. Microcrack accumulation at different intervals during fatigue testing of compact bone. J Biomech. 2003 Jul;36(7):973-80. doi: https://doi.org/10.1016/s0021-9290(03)00066-6
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Copyright (c) 2020 Isabella de Almeida Francisquini, Ricardo Armini Caldas, Gustavo Davi Rabelo
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