Wound healing in weaning, adult, and old rats with provoked incisional hernias. A comparative study

Authors

  • Raphael Nogueira do Amaral Universidade de São Paulo
  • Ana Cristina Aoun Tannuri Universidade de São Paulo
  • Junia Marielle Teixeira Rodrigues Neri Universidade de São Paulo https://orcid.org/0000-0002-1996-8447
  • Hugo de Souza Reis Universidade de São Paulo
  • Josiane Oliveira Gonçalves Universidade de São Paulo
  • Suellen Serafini Universidade de São Paulo
  • Uenis Tannuri Universidade de São Paulo

DOI:

https://doi.org/10.1016/j.clinsp.2022.100106

Abstract

Background: Incisional hernias are more frequent in adults than in children. It is hypothesized that a more efficient healing process in pediatric patients could explain this difference in incidence. Certain elements of healing such as neovascularization, degree of inflammation, percentage of mature and immature collagen, the proliferation of fibroblasts, and expression of certain genes could explain why healing in children is more efficient when compared to the adult and elderly populations.

Materials and methods: Seventy-one rats of 3 different age groups (weaning, adult, and old) underwent surgery with 3 different incisions (vertical, oblique, and horizontal). During the procedure, the skin and abdominal wall of the animal were sectioned and only the skin was sutured to mimic incisional hernia in the animals. Four weeks after surgery, the rats were euthanized, their skin was removed, and the extent of scar tissue formed in the muscle opening was measured. In addition, samples of the scar tissue were collected for histological, immunohistochemical, and molecular analyzes. Nine rats served as controls.

Results: Shorter-length hernias were formed in weaning rats when compared to old ones when the surgical incision was horizontal (p = 0.03). There was a greater proliferation of fibroblasts in rats in the younger age groups, regardless of the type of incision. The Lox gene was more expressed in weaning rats with vertical and oblique incisions.

Conclusions: These differences could explain the better healing and lower incidence of hernias in the pediatric population, although this aspect requires further studies.

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Published

2022-09-19

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Section

Original Articles

How to Cite

Amaral, R. N. do, Tannuri, A. C. A., Neri, J. M. T. R., Reis, H. de S., Gonçalves, J. O., Serafini, S., & Tannuri, U. (2022). Wound healing in weaning, adult, and old rats with provoked incisional hernias. A comparative study. Clinics, 77, 100106. https://doi.org/10.1016/j.clinsp.2022.100106