miR-146b suppresses LPS-induced M1 macrophage polarization via inhibiting the FGL2-activated NF-κB/MAPK signaling pathway in inflammatory bowel disease

Authors

DOI:

https://doi.org/10.1016/j.clinsp.2022.100069

Keywords:

Inflammatory bowel disease, miR-146b, FGL2, Inflammation, M1 Macrophage polarization

Abstract

Objectives: M1 macrophage polarization and phenotype in Inflammatory Bowel Disease (IBD) are common biological responses.

Method: Herein, IBD mice models were constructed and macrophages were derived.

Results: It was discovered that microRNA-146b (miR-146b) was downregulated in IBD mice and Lipopolysaccharide (LPS)-induced macrophages. Moreover, the inhibitory role of overexpressed miR-146b in reducing the inflammation level and blocking M1 macrophage polarization was confirmed. Further investigation indicated that Fibrinogen Like 2 (FGL2) acted as the target gene of miR-146b, and FGL2 mediated activation of NLRP3, NF-κB-p65, and p38-MAPK. More importantly, it was validated that miR-146b could ameliorate inflammatory phenotype and prevent M1 macrophage polarization via inhibiting FGL2 in vitro, and miR-146b overexpression alleviated the intestinal injury of IBD mice in vivo.

Conclusions: Overall, it is potential to use miR-146b for the amelioration of IBD.

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Published

2022-06-21

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Section

Original Articles

How to Cite

Pan, Y., Wang, D., & Liu, F. (2022). miR-146b suppresses LPS-induced M1 macrophage polarization via inhibiting the FGL2-activated NF-κB/MAPK signaling pathway in inflammatory bowel disease. Clinics, 77, 100069. https://doi.org/10.1016/j.clinsp.2022.100069