Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus
DOI:
https://doi.org/10.6061/clinics/2017(02)08Abstract
OBJECTIVES: The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus. METHODS: Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mellitus controls (type 2 diabetes mellitus patients without diabetic retinopathy) were examined by miRNA-specific microarray analysis. Quantitative real-time polymerase chain reaction was used to validate the significantly differentially expressed serum miRNAs from the microarray analysis of 45 diabetic retinopathy cases and 45 age-, sex-, body mass index- and duration-of-diabetes-matched type 2 diabetes mellitus controls. The relative changes in serum miRNA expression levels were analyzed using the 2-ΔΔCt method. RESULTS: A total of 5 diabetic retinopathy cases and 5 type 2 diabetes mellitus controls were included in the miRNA-specific microarray analysis. The serum levels of miR-3939 and miR-1910-3p differed significantly between the two groups in the screening stage; however, quantitative real-time polymerase chain reaction did not reveal significant differences in miRNA expression for 45 diabetic retinopathy cases and their matched type 2 diabetes mellitus controls. CONCLUSION: Our findings indicate that miR-3939 and miR-1910-3p may not play important roles in the development of diabetic retinopathy; however, studies with a larger sample size are needed to confirm our findings.Downloads
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Published
2017-02-01
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Section
Clinical Sciences
How to Cite
Ma, J., Wang, J., Liu, Y., Wang, C., Duan, D., Lu, N., Wang, K., Zhang, L., Gu, K., Chen, S., Zhang, T., You, D., & Han, L. (2017). Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus . Clinics, 72(2), 111-115. https://doi.org/10.6061/clinics/2017(02)08