Acute exposure to hyperosmotic conditions reduces sperm activation by urine in the yellowtail tetra Astyanax altiparanae, a freshwater teleost fish

Authors

  • Nathalia Alcântara Rocha Universidade Estadual de São Paulo, Departamento de Zoologia ; Instituto Chico Mendes de Conservação da Biodiversidade, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental, Laboratório de Biotecnologia de Peixes
  • Gabriel Marra Schade Universidade Estadual de São Paulo, Departamento de Zoologia ; Instituto Chico Mendes de Conservação da Biodiversidade, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental, Laboratório de Biotecnologia de Peixes
  • Álvaro de Miranda Alves Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Claudia de Souza Silva Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Jacqueline Megumi Nakirimoto Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Liura Sanchez Lauri Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Lucca Gobatto Campos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Marcelo Galvão dos Santos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Nicole Nascimento Mesquita Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Rachel Sordi Relvas Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Rafaella Fernandes Carnevale Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Se Yoon Oh Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Victoria Portela Diniz Gaia Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Vivian Renata Kida Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Eneiva Carla Carvalho Celeghini Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Mayra Elena Ortiz D´'Ávila Assumpção Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • José Augusto Senhorini Universidade Estadual de São Paulo, Departamento de Zoologia ; Instituto Chico Mendes de Conservação da Biodiversidade, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental, Laboratório de Biotecnologia de Peixes
  • Hatus de Oliveira Siqueira Universidade Estadual de São Paulo, Departamento de Zoologia ; Instituto Chico Mendes de Conservação da Biodiversidade, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental, Laboratório de Biotecnologia de Peixes
  • Nivaldo Ferreira Nascimento Instituto Chico Mendes de Conservação da Biodiversidade, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental, Laboratório de Biotecnologia de Peixes ; Universidade Estadual de São Paulo, Centro de Aquicultura
  • Luciano Andrade Silva Universidade de São Paulo, Faculdade de Medicina Veterinária e Ciência Animal, Departamento de Reprodução Animal
  • Talita Maria Lázaro Instituto Chico Mendes de Conservação da Biodiversidade, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental, Laboratório de Biotecnologia de Peixes
  • José Antonio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Paulo Sérgio Monzani Instituto Chico Mendes de Conservação da Biodiversidade, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental, Laboratório de Biotecnologia de Peixes
  • George Shigueki Yasui Universidade Estadual de São Paulo, Departamento de Zoologia ; Instituto Chico Mendes de Conservação da Biodiversidade, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental, Laboratório de Biotecnologia de Peixes ; Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal https://orcid.org/0000-0002-6105-8308

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2020.166205

Keywords:

Fish, Urospermia, Sperm contamination, Salinity

Abstract

In freshwater fish with external fertilization, sperm sampling can be contaminated with urine, which triggers motility and gives rise to decreased fertilization success. The maintenance of freshwater fish in hyperosmotic conditions may reduce urine production and improve sperm quality. Thus, the aim of this work was to verify if acute exposure to various NaCl concentrations improves sperm quality in the yellowtail tetra Astyanax altiparanae. Spermiation was induced using a single dose of carp pituitary gland (5 mg kg-1) and the males were maintained at various NaCl concentrations: NaCl 0.00% (control), NaCl 0.45% (hypoosmotic), NaCl 0.9% (isosmotic) and NaCl 1.0% (hyperosmotic) for 6 h at 26 °C. Sperm was collected and verified for activation by urine and motility traits. At 0.00%, 0.45%, and 0.90%, the sperm was motile just after sampling, indicating activation by urine. Surprisingly, at hyperosmotic conditions, no activation was observed. Other sperm and motility parameters did not show any statistical differences, including sperm viability (P = 0.7083), concentration (P = 0.9030), total motility (P = 0.6149), VCL (curvilinear velocity; P = 0.1216), VAP (average path velocity; P = 0.1231) and VSL (straight-line velocity; P = 0.1340). Our results indicate that acute maintenance at hyperosmotic conditions eliminates sperm activation by urine and maintains sperm quality. Such a new procedure is interesting for both basic and applied sciences, including reproductive practice in fish.

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References

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2020-10-07

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Vol. 57 No. 3 (2020)

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How to Cite

1.
Rocha NA, Schade GM, Alves Álvaro de M, Silva C de S, Nakirimoto JM, Lauri LS, et al. Acute exposure to hyperosmotic conditions reduces sperm activation by urine in the yellowtail tetra Astyanax altiparanae, a freshwater teleost fish. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2020 Oct. 7 [cited 2024 Dec. 11];57(3):e166205. Available from: https://journals.usp.br/bjvras/article/view/166205