Isolation of canine parvovirus in Brazil

Abstract

The canine parvovirus was isolated from 24 samples of faeces, one mucosal scraping, one vomiting material obtained from dogs that had presented signs of hemorrhagic enteritis, and from one sample of faeces from a normal dog. All the materials were previously tested by hemagglutination reaction with swine red cells. There is an agreement of 96,3% between this reaction and isolation of parvovirus cell cultures, demonstrating the great practical utility of the hemagglutination reaction as a trial method for the demonstration of the presence of parvovirus in the stool of infected dogs . Electron microscopy of infected cells demonstrated the presence of viral particles with parvovirus morphology and the hemagglutination inhibition and immunofluorescence reactions, using feline parvovirus immune serum, revealed the antigenic relationship between the isolate and feline parvovirus.