In vitro cultivation of Ascaris suum larvae: simplification of the culture medium

Authors

  • Jorge Guerrero Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • M. L.P. Mercadante Faculdade de Medicina do ABC, Santo André, SP

DOI:

https://doi.org/10.11606/issn.2318-3659.v13i2p391-399

Keywords:

Ascaris suum, In vitro culture, Column chromatography, Electroplioresis (supplements)

Abstract

Attempts were made to simplify the culture medium utilized for in vitro cultivation of A. suum larvae. Of all the supplements utilized to try to replace the fetal calf serum it was found that hemin plus any other protein supplement considerably increased both the larval survival and rate of developments. Egg albumin used with hemin or glicogen gave excelent results as a suppement to EAGLE'S minimum essential medium utilized in the in vitro culture of A. suum larvae.

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Published

1976-09-15

Issue

Vol. 13 No. 2 (1976)

Section

ORIGINAL ARTICLES

How to Cite

In vitro cultivation of Ascaris suum larvae: simplification of the culture medium. (1976). Revista Da Faculdade De Medicina Veterinária E Zootecnia Da Universidade De São Paulo, 13(2), 391-399. https://doi.org/10.11606/issn.2318-3659.v13i2p391-399