Human chondrocytes culture to treatement of cartilaginous injuries

Abstract

This work presents as objective to test the culture of chondrocytes for a not enzymatic method. For this, they had been isolated chondrocytes of costal cartilage human being for the method of primary explante, where the cells migrate of the cartilage and if they had adhered to the deep one in the bottle of culture. Later, for the method of indirect immunofluorescence it was dosed capacity of production of collagen type II for the isolated chondrocytes in order to evaluate the capacity of the cells to produce extracellular matrix to demonstrate the maintenance of the physiological capacities of the isolated cells in cullture.For bioengineering confides possibility to create cartilage in vitro to substitute one fabric injured, which is not capable of if regenerating by your own. The explante is a simple and cheap method that allows the isolation of human chondrocytes. One proved that the cultivated chondrocytes keep its phenotypic capacity to produce collagen type II. In this way, one proves that the isolated cells produce extracellular matrix typical of the fabric cartilaginous and are potentially usable for treatment of cartilaginous injuries