Temporo-spatial distribution of stem cell markers CD146 and p75NTR during odontogenesis in mice

Authors

  • Aline Queiroz Universidade de São Paulo, Faculdade de Odontologia, Departamento de Estomatologia, Disciplina de Patologia Oral e Maxilofacial, Laboratório de Biologia de Células-Tronco em Odontologia LABITRON, São Paulo http://orcid.org/0000-0002-9564-2318
  • Cibele Pelissari Universidade de São Paulo, Faculdade de Odontologia, Departamento de Estomatologia, Disciplina de Patologia Oral e Maxilofacial, Laboratório de Biologia de Células-Tronco em Odontologia LABITRON, São Paulo, SP http://orcid.org/0000-0003-0067-071X
  • Victor Elias Arana-Chavez Universidade de São Paulo, Faculdade de Odontologia, Departamento de Biomateriais e Biologia Oral, São Paulo http://orcid.org/0000-0002-6767-7812
  • Marília Trierveiler Universidade de São Paulo, Faculdade de Odontologia, Departamento de Estomatologia, Disciplina de Patologia Oral e Maxilofacial, Laboratório de Biologia de Células-Tronco em Odontologia LABITRON, São Paulo http://orcid.org/0000-0002-8517-2105

DOI:

https://doi.org/10.1590/1678-7757-2021-0138

Keywords:

Tooth development, Odontogenic stem cells, Odontogenesis, ; p75 neurotrophin receptor, CD146 antigen

Abstract

Mesenchymal and epithelial stem cells were identified in dental tissues; however, knowledge about the odontogenic stem cells is limited, and there are some questions regarding their temporo-spatial dynamics in tooth development. Objective: Our study aimed to analyze the expression of the stem cell markers CD146 and p75NTR during the different stages of odontogenesis. Methodology: The groups consisted of 13.5, 15.5, 17.5 days old embryos, and 14 days postnatal BALB/c mice. The expression of CD146 and p75NTR was evaluated by immunohistochemistry. Results: Our results showed that positive cells for both markers were present in all stages of tooth development, and the number of positive cells increased with the progression of this process. Cells of epithelial and ectomesenchymal origin were positive for CD146, and the expression of p75NTR was mainly detected in the dental papilla and dental follicle. In the postnatal group, dental pulp cells were positive for CD146, and the reduced enamel epithelium and the oral mucosa epithelium showed immunostaining for p75NTR. Conclusions: These results suggest that the staining pattern of CD146 and p75NTR underwent temporal and spatial changes during odontogenesis and both markers were expressed by epithelial and mesenchymal cell types, which is relevant due to the significance of the epithelial-ectomesenchymal interactions in tooth development.

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Published

2021-10-13

Issue

Vol. 29 (2021)

Section

Original Articles

License

Copyright (c) 2021 Journal of Applied Oral Science

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How to Cite

Queiroz, A., Pelissari, C., Arana-Chavez, V. E., & Trierveiler, M. (2021). Temporo-spatial distribution of stem cell markers CD146 and p75NTR during odontogenesis in mice. Journal of Applied Oral Science, 29, e20210138. https://doi.org/10.1590/1678-7757-2021-0138