Laboratory model to evaluate the influence of stress and corrosion in the formation of non-carious cervical lesions

Authors

  • Noélia Maria de Sousa Leal Department of Morphology, Health Sciences Center, Federal University of Piauí, Teresina, PI, Brazil
  • Maria Ivone Mendes Benigno Department of Morphology, Health Sciences Center, Federal University of Piauí, Teresina, PI, Brazil
  • Juscelino Lopes Silva Department of Morphology, Integral Differential College, Teresina, PI, Brazil
  • Simone Soares Pedrosa Department of Prosthodontics, School of Dentistry, Federal University of Pará, Belém, PA, Brazil
  • Eliane Bemerguy Alves Department of Restorative Dentistry, School of Dentistry, Federal University of Pará, Belém, PA, Brazil
  • Rafael Yagüe Ballester Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil

DOI:

https://doi.org/10.11606/issn.2357-8041.v20i2p117-124

Keywords:

Demineralization, Tensile Strength, Dental Enamel, Dentin.

Abstract

Objective: To conceive and test a laboratory model that simulates the multifactorial etiology of non-carious cervical lesions. The model enables researchers to assess the pathological process with increasing levels of complexity, focusing on tension and corrosion. The model is aimed at clarifying the mechanisms that lead to the development of these lesions. Methods: Specimens were manufactured from bovine incisors cut into 18 × 3 × 3 mm sticks, with a notch in the cervical region facing the pulp, in order to concentrate the stresses on the surface of the cementum-enamel junction when fi xed at the apical end and loaded for bending on the incisal edge. One group was immersed in distilled water and the other in pH 4.5 acetate buffer for 72 h. Each group was divided into three subgroups: one subgroup without loading, and two subgroups submitted to loading (800 gf) to cause either compression or tensile stress. After the test, 0.05 mm histological lamellae of the specimens were processed and photographed using a light microscope, and the damages were assessed. Conclusion: The laboratory model that was developed enabled the precise measurement of the depth of loss and demineralization of tooth tissue in the specimens, whether submitted to stress or not. The formation of other damages, such as cracks and fractures, could also be observed; this made it possible to infer the infl uence of compressive and tensile stresses on the etiology of non-carious cervical lesions. The model can be further enhanced by making it possible to apply cyclic loads and interspersed abrasive challenges.

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Author Biographies

  • Noélia Maria de Sousa Leal, Department of Morphology, Health Sciences Center, Federal University of Piauí, Teresina, PI, Brazil
    Professora Adjunta do Departamento de Morfologia da UFPI e Doutoranda na área de Biomateriais e Biologia Oral na USP
  • Maria Ivone Mendes Benigno, Department of Morphology, Health Sciences Center, Federal University of Piauí, Teresina, PI, Brazil
    Professora Adjunta do Departamento de Morfologia da UFPI e Doutoranda em Ciências Médicas na Unicamp
  • Juscelino Lopes Silva, Department of Morphology, Integral Differential College, Teresina, PI, Brazil
    Professor de Morfologia da Faculdade Integral Diferencial
  • Simone Soares Pedrosa, Department of Prosthodontics, School of Dentistry, Federal University of Pará, Belém, PA, Brazil
    Professora Adjunta da Universidade Federal do Pará e Doutoranda na área de Biomateriais e Biologia Oral na USP
  • Eliane Bemerguy Alves, Department of Restorative Dentistry, School of Dentistry, Federal University of Pará, Belém, PA, Brazil
    Professora Adjunta da Universidade Federal do Pará
  • Rafael Yagüe Ballester, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil
    Professor Titular da Universidade de São Paulo e Coordenador do Programa de Pós-Graduação em Materiais Dentários

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Published

2014-03-14

Issue

Vol. 20 No. 2 (2014)

Section

Case report or technical report

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How to Cite

Leal, N. M. de S., Benigno, M. I. M., Silva, J. L., Pedrosa, S. S., Alves, E. B., & Ballester, R. Y. (2014). Laboratory model to evaluate the influence of stress and corrosion in the formation of non-carious cervical lesions. Clinical and Laboratorial Research in Dentistry, 20(2), 117-124. https://doi.org/10.11606/issn.2357-8041.v20i2p117-124