Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation

Authors

  • Gisele Negro Lima Paulista School of Medicine at Federal University of São Paulo (EPM/UNIFESP); Department of Gynecology
  • Carla Cristina Maganhin Paulista School of Medicine at Federal University of São Paulo (EPM/UNIFESP); Department of Gynecology
  • Ricardo Santos Simões Faculdade de Medicina da Universidade de São Paulo (FMUSP/USP); Department of Obstetrics and Gynecology
  • Maria Cândida Pinheiro Baracat Faculdade de Medicina da Universidade de São Paulo (FMUSP/USP); Department of Obstetrics and Gynecology
  • Gisela Rodrigues da Silva Sasso Paulista School of Medicine at Federal University of São Paulo; (EPM/UNIFESP); Department of Morphology and Genetics
  • Luiz Fernando Portugal Fuchs Faculdade de Medicina da Universidade de São Paulo (FMUSP/USP); Department of Obstetrics and Gynecology
  • Manuel de Jesus Simões Paulista School of Medicine at Federal University of São Paulo; (EPM/UNIFESP); Department of Morphology and Genetics
  • Edmund Chada Baracat Faculdade de Medicina da Universidade de São Paulo (FMUSP/USP); Department of Obstetrics and Gynecology
  • José Maria Soares Júnior Faculdade de Medicina da Universidade de São Paulo (FMUSP/USP); Department of Obstetrics and Gynecology

DOI:

https://doi.org/10.6061/clinics/2015(02)12

Abstract

OBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 µg/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip¯ Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip¯ Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis.

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Published

2015-02-01

Issue

Section

Basic Researchs

How to Cite

Lima, G. N., Maganhin, C. C., Simões, R. S., Baracat, M. C. P., Sasso, G. R. da S., Fuchs, L. F. P., Simões, M. de J., Baracat, E. C., & Soares Júnior, J. M. (2015). Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation . Clinics, 70(2), 144-151. https://doi.org/10.6061/clinics/2015(02)12