Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels

Authors

  • Joycellane Alline do Nascimento Campos Ribeiro Federal University of Rio Grande do Norte; Biosciences Center; Department of Biochemistry
  • Alexandre Coellho Serquiz Federal University of Rio Grande do Norte; Biosciences Center; Department of Biochemistry
  • Priscila Fabíola dos Santos Silva Federal University of Rio Grande do Norte; Center for Health Sciences; Department of Nutrition, Rio Grande do Norte
  • Patrícia Batista Barra Medeiros Barbosa Federal University of Rio Grande do Norte; Biosciences Center; Department of Biochemistry
  • Tarcísio Bruno Montenegro Sampaio Potiguar University; Biology Course
  • Raimundo Fernandes de Araújo Junior Federal University of Rio Grande do Norte; Center for Biosciences; Department of Morfology
  • Adeliana Silva de Oliveira Federal University of Rio Grande do Norte; Biosciences Center; Department of Biochemistry
  • Richele Janaina Araújo Machado Federal University of Rio Grande do Norte; Biosciences Center; Department of Biochemistry
  • Bruna Leal Lima Maciel Federal University of Rio Grande do Norte; Center for Health Sciences; Department of Nutrition, Rio Grande do Norte
  • Adriana Ferreira Uchôa Federal University of Rio Grande do Norte; Center for Biosciences; Department of Cell Biology and Genetics
  • Elizeu Antunes dos Santos Federal University of Rio Grande do Norte; Biosciences Center; Department of Biochemistry
  • Ana Heloneida de Araújo Morais Federal University of Rio Grande do Norte; Center for Health Sciences; Department of Nutrition, Rio Grande do Norte

DOI:

https://doi.org/10.6061/clinics/2015(02)11

Abstract

OBJECTIVES: Seeds are excellent sources of proteinase inhibitors, some of which may have satietogenic and slimming actions. We evaluated the effect of a trypsin inhibitor from Tamarindus indica L. seeds on weight gain, food consumption and cholecystokinin levels in Wistar rats. METHODS: A trypsin inhibitor from Tamarindus was isolated using ammonium sulfate (30-60%) following precipitation with acetone and was further isolated with Trypsin-Sepharose affinity chromatography. Analyses were conducted to assess the in vivo digestibility, food intake, body weight evolution and cholecystokinin levels in Wistar rats. Histological analyses of organs and biochemical analyses of sera were performed. RESULTS: The trypsin inhibitor from Tamarindus reduced food consumption, thereby reducing weight gain. The in vivo true digestibility was not significantly different between the control and Tamarindus trypsin inhibitor-treated groups. The trypsin inhibitor from Tamarindus did not cause alterations in biochemical parameters or liver, stomach, intestine or pancreas histology. Rats treated with the trypsin inhibitor showed significantly elevated cholecystokinin levels compared with animals receiving casein or water. CONCLUSION: The results indicate that the isolated trypsin inhibitor from Tamarindus reduces weight gain by reducing food consumption, an effect that may be mediated by increased cholecystokinin. Thus, the potential use of this trypsin inhibitor in obesity prevention and/or treatment should be evaluated.

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Published

2015-02-01

Issue

Section

Basic Researchs

How to Cite

Ribeiro, J. A. do N. C., Serquiz, A. C., Silva, P. F. dos S., Barbosa, P. B. B. M., Sampaio, T. B. M., Araújo Junior, R. F. de, Oliveira, A. S. de, Machado, R. J. A., Maciel, B. L. L., Uchôa, A. F., Santos, E. A. dos, & Morais, A. H. de A. (2015). Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels . Clinics, 70(2), 136-143. https://doi.org/10.6061/clinics/2015(02)11