The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients

Authors

  • Ali Sheikh Md Sayed Central South University; Xiangya Hospital; Department of Cardiology; Central South University
  • Ke Xia Harvard Medical School; Brigham and Women's Hospital; Center for Vascular Biology and Inflammation
  • Fei Li Central South University; Xiangya Hospital; Department of Cardiology; Central South University
  • Xu Deng Central South University; Xiangya Hospital; Department of Cardiology; Central South University
  • Umme Salma Central South University; Xiangya 3rd Hospital; Department of Gynecology and Obstetrics; Central South University
  • Tingbo Li Central South University; School of Pharmaceutical Sciences; Department of Pharmacology; Central South University
  • Hai Deng Central South University; Xiangya Hospital; Department of Cardiology; Central South University
  • Dafeng Yang Central South University; Xiangya Hospital; Department of Cardiology; Central South University
  • Zhou Haoyang Central South University; Xiangya Hospital; Department of Cardiology; Central South University
  • TianLun Yang Central South University; Xiangya Hospital; Department of Cardiology; Central South University
  • Jun Peng Central South University; School of Pharmaceutical Sciences; Department of Pharmacology; Central South University

DOI:

https://doi.org/10.6061/clinics/2015(04)07

Abstract

OBJECTIVE: Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-year-old) patients. METHODS: Sixty-five stable coronary artery disease patients (49–57 years old), 30 unstable coronary artery disease patients (49–58 years old), and 32 non-coronary artery disease patients (49–-57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction. RESULTS: Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (p<0.001). Circulating miR-424 levels were reduced 3.6-fold in stable coronary artery disease patients (1.18 ± 0.60) and 5-fold in unstable coronary artery disease patients (0.86 ± 0.54) compared with non-coronary artery disease patients (4.35 ± 2.20) (p<0.001). In contrast, circulating miR-765 levels were elevated 3.98-fold in stable coronary artery disease patients (6.09 ± 2.27) and 5.33-fold in unstable coronary artery disease patients (8.17 ± 2.77) compared with non-coronary artery disease patients (1.53 ± 0.99) (p<0.001). Receiver operating characteristic curve analysis revealed that the respective areas under the curve for circulating miR-149, miR-424 and miR-765 were 0.938, 0.919 and 0.968 in stable CAD patients and 0.951, 0.960 and 0.977 in unstable coronary artery disease patients compared with non-coronary artery disease patients. CONCLUSION: Our results suggest that circulating miR-149, miR-424 and miR-765 might be novel, non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged patients. However, future prospective trials in large patient cohorts are necessary before reaching a solid conclusion.

Downloads

Download data is not yet available.

Downloads

Published

2015-04-01

Issue

Section

Clinical Sciences

How to Cite

Sayed, A. S. M., Xia, K., Li, F., Deng, X., Salma, U., Li, T., Deng, H., Yang, D., Haoyang, Z., Yang, T., & Peng, J. (2015). The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients . Clinics, 70(4), 257-263. https://doi.org/10.6061/clinics/2015(04)07