Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Authors

  • Thalyta Marina Benetti Federal University of Paraná; Department of Basic Pathology
  • Cristina Leise Bastos Monteiro Federal University of Paraná; Department of Basic Pathology
  • Márcia Regina Beux Federal University of Paraná; Department of Basic Pathology
  • Wanda Moscalewski Abrahão Paraná State Central Laboratory; Section of Food Microbiology

DOI:

https://doi.org/10.1590/S1984-82502012000400011

Keywords:

Listeria sp.^i1^sdetect, Listeria sp.^i1^smeat produ, Meat products^i1^squalitative analy, Refrigerated sausages^i1^squalitative analy, PALCAM Agar, Oxford Agar, Chromogenic Agar

Abstract

The aim of this study was to determine the prevalence of Listeria sp. in refrigerated sausages, and to compare the performance of the selective plating media employed in the ISO 11290-1 method (PALCAM and Oxford agars) with chromogenic agars (Chromogenic Listeria agars CM 1080 (OCLA) and CM 1084). The prevalence of Listeria sp. detected was 52.9%, comprising 13.7% L. monocytogenes strains. The efficacy of the four agars for the isolation of L. monocytogenes proved to be satisfactory. Despite differences in composition of the chromogenic media assessed, these disparities did not affect concordance among results. However, PALCAM agar was shown to suppress other microorganisms more effectively, being more applicable for detecting Listeria strains present in lower quantities. Based on these results, the use of PALCAM agar, in combination with a chromogenic media, is recommended for enhanced isolation of atypical Listeria sp. strains in meat products.

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Published

2012-12-01

Issue

Vol. 48 No. 4 (2012)

Section

Articles

License

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

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How to Cite

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages . (2012). Brazilian Journal of Pharmaceutical Sciences, 48(4), 677-681. https://doi.org/10.1590/S1984-82502012000400011