Quality of commercialized dry extracts of blood orange juice in Brazilian compounding pharmacies: Dry extracts of blood orange juice

Lídia Cristina Merlin de  Meneses; Maria Izabel  Lamego Neta; Jhenifer Luisa  Miniuki; Ingrid Vicente  Farias; Larissa  Benvenutti; Otto Mauricio Santos  Gerlach; Flávio H.  Reginatto; Angela  Malheiros; José Roberto  Santin; Tania Mari Bellé  Bresolin

doi:10.1590/

Authors

Lídia Cristina Merlin de Meneses Postgraduate Program in Pharmaceutical Sciences; Universidade do Vale do Itajaí, UNIVALI, Itajaí, SC, Brazil
Maria Izabel Lamego Neta Biomedicine Program, Universidade do Vale do Itajaí, UNIVALI, Itajaí, SC, Brazil
Jhenifer Luisa Miniuki Postgraduate Program in Pharmaceutical Sciences; Universidade do Vale do Itajaí, UNIVALI, Itajaí, SC, Brazil
Ingrid Vicente Farias Department of Pharmaceutical Sciences, Health Sciences Center, Universidade Federal Santa Catarina, UFSC, Florianópolis, SC, Brazil
Larissa Benvenutti Postgraduate Program in Pharmaceutical Sciences; Universidade do Vale do Itajaí, UNIVALI, Itajaí, SC, Brazil
Otto Mauricio Santos Gerlach Postgraduate Program in Pharmaceutical Sciences; Universidade do Vale do Itajaí, UNIVALI, Itajaí, SC, Brazil
Flávio H. Reginatto Department of Pharmaceutical Sciences, Health Sciences Center, Universidade Federal Santa Catarina, UFSC, Florianópolis, SC, Brazil
Angela Malheiros Postgraduate Program in Pharmaceutical Sciences; Universidade do Vale do Itajaí, UNIVALI, Itajaí, SC, Brazil
José Roberto Santin Postgraduate Program in Pharmaceutical Sciences; Universidade do Vale do Itajaí, UNIVALI, Itajaí, SC, Brazil
Tania Mari Bellé Bresolin Postgraduate Program in Pharmaceutical Sciences; Universidade do Vale do Itajaí, UNIVALI, Itajaí, SC, Brazil https://orcid.org/0000-0002-4200-9201

DOI:

https://doi.org/10.1590/

Keywords:

Anthocyanins, Citrus sinensis, Antioxidant, Anti-inflammatory

Abstract

The juice of blood oranges, especially those of the Citrus sinensis variety Moro, cultivated in the region of Sicily (Italy), is an increasingly popular drink due to its beneficial health properties, such as its ability to reduce abdominal fat, related to its anthocyanin constituents, especially cyanidin-3-O-glycoside (C3G), as demonstrated in pre-clinical and clinical studies. However, the dry extract of C. sinensis juice currently available at compounding pharmacies in Brazil includes samples from various countries, some of which may not have adequate climatic conditions for the production of anthocyanins. In this work, we investigated samples from the three major suppliers (reference, A1, and A2). The composition of the samples was analyzed by LC-UV and LC-MS, total anthocyanin content (TAC), antioxidant activity (DPPH assay), and in vitro anti-inflammatory effect, by NO production in macrophages. C3G was detected only in the reference sample (1.6%), the TAC values were 1.45%, 0.1% and 0.01% in the reference, A1, and A2, respectively. The reference and A1 showed similar antioxidant activity (EC50 of 45.6 and 62.4 µg/mL, respectively), while A2 showed lower activity (EC₅₀ 315.1 µg/mL). Only the reference sample showed significant inhibition of NO release, demonstrating the need for quality control of these commercialized samples.

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Quality of commercialized dry extracts of blood orange juice in Brazilian compounding pharmacies

Dry extracts of blood orange juice

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