A novel nano-emulsion cream of casein isolated from raw milk for thermal and acid burn wounds

Shivani  Shrivastava; Saumya  Das; Chanchal  Sharma; Archna  Singh; Sanjita  Das; Manas Kumar Das; Pankaj Kumar  Tyagi; Navneet  Khurana

doi:10.1590/

Authors

Shivani Shrivastava Noida Institute of Engineering and Technology (Pharmacy Institute), Greater Noida, Uttar Pradesh, India
Saumya Das Noida Institute of Engineering and Technology (Pharmacy Institute), Greater Noida, Uttar Pradesh, India https://orcid.org/0000-0002-8531-9963
Chanchal Sharma Noida Institute of Engineering and Technology (Pharmacy Institute), Greater Noida, Uttar Pradesh, India
Archna Singh Noida Institute of Engineering and Technology (Pharmacy Institute), Greater Noida, Uttar Pradesh, India https://orcid.org/0009-0003-4109-9158
Sanjita Das Department of Pharmacy, SMAS, Galgotias University, Greater Noida, Uttar Pradesh, India https://orcid.org/0000-0002-9863-9341
Manas Kumar Das Orlean College of Pharmacy, Greater Noida, Uttar Pradesh, India https://orcid.org/0000-0002-9785-8905
Pankaj Kumar Tyagi Department of Biotechnology, Noida Institute of Engineering and Technology, Greater Noida, Uttar Pradesh, India https://orcid.org/0000-0002-7003-0489
Navneet Khurana Department of Pharmaceutical Sciences, Lovely Professional University, Punjab, India https://orcid.org/0000-0001-9899-4856

DOI:

https://doi.org/10.1590/

Keywords:

Acid burn, Casein, Cream, Hydroxyproline, Nanoemulsion, Raw milk, Thermal wound

Abstract

Traditionally, raw cow milk has been employed to treat acid and thermal burns due to its antibacterial, antibiotic, and antifungal properties. It also aids in preventing scarring and alleviating burn-related skin discomfort. This study focuses on evaluating a nanoemulsion cream made from casein extracted from raw cow milk for managing acid and thermal burns. Casein was isolated and used to create 2%, 5%, and 10% w/w nanoemulsion creams. Wistar albino rats were used to induce acid and thermal burns, and the healing progress was monitored by measuring wound contraction and epithelialization period. The results demonstrated significant wound healing effects of the casein creams (p<0.001) in acid burns, with the 10% w/w cream showing the highest epithelization rate in thermal burns. Raw milk also exhibited notable healing properties in acid burns. However, its effectiveness was relatively lower in thermal burns. This study suggests that raw milk and its casein-derived creams could be utilized as first-aid treatments for acid burns, though they may be less effective for thermal burns.

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A novel nano-emulsion cream of casein isolated from raw milk for thermal and acid burn wounds

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