Plantago major leaf extract against bacteria and fungi of medical importance

Authors

  • Débora Silva Marques de Sousa Júlio de Mesquita Filho State University of São Paulo (UNESP), Department of Chemical and Biological Sciences, Microbiology and Immunology Sector, Botucatu, São Paulo, Brazil https://orcid.org/0000-0002-7301-7909
  • Luciana da Silva Ruiz Adolfo Lutz Institute, São Paulo, Brazil https://orcid.org/0000-0001-7374-8640
  • Ana Flávia Marques Pereira Júlio de Mesquita Filho State University of São Paulo (UNESP), Department of Chemical and Biological Sciences, Microbiology and Immunology Sector, Botucatu, São Paulo, Brazil https://orcid.org/0000-0002-2925-8545
  • Alessandra Aguirra Sani Júlio de Mesquita Filho State University of São Paulo (UNESP), Department of Chemical and Biological Sciences, Microbiology and Immunology Sector, Botucatu, São Paulo, Brazil https://orcid.org/0000-0002-0828-9799
  • Tatiane Baptista Zapata Júlio de Mesquita Filho State University of São Paulo (UNESP), Department of Chemical and Biological Sciences, Microbiology and Immunology Sector, Botucatu, São Paulo, Brazil https://orcid.org/0000-0002-7653-184X
  • Gabrielle Pires de Morais Monari Adolfo Lutz Institute, São Paulo, Brazil https://orcid.org/0009-0008-5222-8417
  • Cassiana Ferreira da Rosa Adolfo Lutz Institute, São Paulo, Brazil
  • Ary Fernandes Junior Júlio de Mesquita Filho State University of São Paulo (UNESP), Department of Chemical and Biological Sciences, Microbiology and Immunology Sector, Botucatu, São Paulo, Brazil https://orcid.org/0000-0002-2744-2824
  • Vera Lucia Mores Rall Júlio de Mesquita Filho State University of São Paulo (UNESP), Department of Chemical and Biological Sciences, Microbiology and Immunology Sector, Botucatu, São Paulo, Brazil https://orcid.org/0000-0003-4280-5619
  • Diego Godina Prado Federal University of Uberlandia, Institute of Chemistry, Federal University of Uberlândia, Santa Mônica Campus Uberlândia, MG, Minas Gerais, Brazil https://orcid.org/0000-0002-7192-7765
  • Alberto de Oliveira Federal University of Uberlandia, Institute of Chemistry, Federal University of Uberlândia, Santa Mônica Campus Uberlândia, MG, Minas Gerais, Brazil https://orcid.org/0000-0002-7210-164X
  • Sandra de Moraes Gimenes Bosco Faculdade Galileu, Botucatu, São Paulo, Brazil https://orcid.org/0000-0002-6363-3740

DOI:

https://doi.org/10.1590/

Keywords:

Synergistic interaction, Natural products, Multi-drug resistant microorganisms, Antimicrobial drugs, Extract chemical composition, Mass spectrometry

Abstract

Antimicrobial drug resistance is a challenge to public health. Various microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, carbapenemase-producing Klebsiella pneumoniae, Pythium insidiosum, and Candida auris, have developed resistance to commonly used antimicrobials in infection disease treatments. Consequently, there is an urgent need to explore and develop novel therapeutic drugs. Natural products, particularly medicinal plants, have received attention in the quest for innovative treatments for various diseases. Plantago major is a plant endowed with several biological properties, such as antibacterial, antifungal, and healing actions. This study aimed to investigate the antimicrobial activities of the methanolic extract obtained from the leaves of P. major. Microdilution assays were conducted to determine the minimum inhibitory concentration and minimum bactericidal and fungicidal concentration. Additionally, synergism with antimicrobial drugs was assessed using a time-kill curve analysis. A synergistic bactericidal interaction between the extract and imipenem was observed against carbapenemase-producing K. pneumoniae. For MRSA, a bacteriostatic synergism was noted in combinations of the extract with cephalotin and oxacillin. For C. auris, a fungistatic interaction was observed between the extract and amphotericin B. These results suggest the presence of bioactive compounds within the extract with therapeutic potential for combating infections caused by these microorganisms.

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Published

2025-02-11

Issue

Vol. 61 (2025)

Section

Article

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How to Cite

Plantago major leaf extract against bacteria and fungi of medical importance. (2025). Brazilian Journal of Pharmaceutical Sciences, 61. https://doi.org/10.1590/

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