Vasoconstrictive effects of betanin on isolated preparations of rat aorta
DOI:
https://doi.org/10.1590/Keywords:
Betanin, Contractile effect, Endothelium function, Vascular smooth muscleAbstract
Betanin is a natural pigment belonging to the group of betalains with vasoconstrictive properties. This study investigated the underlying mechanism involved in the vasoconstrictive effect of betanin in rat aorta. Betanin enhanced the contractile response in aortic rings precontracted with phenylephrine. In aortic preparations under resting tonus, betanin induced potent and sustained contractions. The contractile effect of betanin was increased in endothelium-denuded aortic rings, while treatment of endothelium-intact preparations with the alpha-1-adrenergic receptor antagonist prazosin abolished the contractile effects of betanin. In the presence of verapamil, the contractile effects of betanin were decreased, and they were abolished under Ca 2+ -free solution. Vasoconstriction was not affected by treatment with yohimbine and guanethidine. Betanin partially restored the endothelium-dependent vasorelaxant effect induced by acetylcholine, a response that was blunted in aortic rings treated with baicalin, an inhibitor of the nitric oxide (NO) - cyclic guanosine monophosphate (cGMP) - protein kinase G (PKG) pathway. In conclusion, the vasoconstrictive effects of betanin likely involve alpha-1 adrenoceptors and are dependent on endothelial modulation.
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