Compound Danshen Dripping Pills pretreatment protects the heart from ischemia/reperfusion injury by enhancing autophagic flux

Authors

  • Haiqiong Liu Southern Medical University. Zhujiang Hospital
  • Qian Liang The First People’s Hospital of Foshan City
  • Xiheng Mei Southern Medical University. Zhujiang Hospital. Zhujiang Hospital of Southern Medical University
  • Hekai Li Li Southern Medical University. Zhujiang Hospital
  • Jing Yan Southern Medical University. Zhujiang Hospital. Zhujiang Hospital of Southern Medical University
  • Man Long Ultrasound Medical Center, Zhujiang Hospital of Southern Medical University, China
  • Xili Yang Department of Cardiology of The First People’s Hospital of Foshan City, China
  • Wei Wang Department of Cardiology of The First People’s Hospital of Foshan City, China
  • Weijie Li Department of Cardiology of The First People’s Hospital of Foshan City, China
  • Aihua Chen Department of Health Management, Zhujiang Hospital, Southern Medical University, China http://orcid.org/0000-0002-7682-4255
  • Yuanna Ling Department of Nuclear Medicine of Zhujiang Hospital of Southern Medical University, China http://orcid.org/0000-0002-2282-0718

DOI:

https://doi.org/10.1590/s2175-97902023e211035

Keywords:

ischemia/reperfusion;, Compound Danshen Dripping Pills;, Autophagy;, Apoptosis

Abstract

Compound Danshen Dripping Pills (CDDPs) have been used in clinical treatment to protect the heart from ischemia/reperfusion (IR) injury for many years. However, the underlying mechanism implicated in the protective effects remains to be explored. Here, we determined the effects of CDDPs in Sprague-Dawley rats with the IR model. Cardiac function in vivo was assessed by echocardiography. Transmission electron microscopy, histological and immunohistochemical techniques, Western blotting and recombinant adeno-associated virus 9 transfection were used to illustrate the effects of CDDPs on IR and autophagy. Our results showed that pretreatment with CDDPs decreased the level of serum myocardial enzymes and infarct size in rats after IR. Apoptosis evaluation showed that CDDPs significantly ameliorated the cardiac apoptosis level after IR. Meanwhile, CDDPs pretreatment increased myocardial autophagic flux, with upregulation of LC3B, downregulation of p62, and increased autophagosomes and autolysosomes. Moreover, the autophagic flux inhibitor chloroquine could increase IR injury, while CDDPs could partially reverse the effects. Furthermore, our results showed that the activation of AMPK/mTOR was involved in the cardioprotective effect exerted by CDDPs. Herein, we suggest that CDDPs partially protect the heart from IR injury by enhancing autophagic flux through the activation of AMPK/mTOR.

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Author Biographies

  • Haiqiong Liu, Southern Medical University. Zhujiang Hospital
    writing-original Draft investigation revising manuscript Haiqiong Liu and Qian Liang contributed equally to this article.
  • Qian Liang, The First People’s Hospital of Foshan City
    conceptualization methodology Haiqiong Liu and Qian Liang contributed equally to this article.
  • Xiheng Mei, Southern Medical University. Zhujiang Hospital. Zhujiang Hospital of Southern Medical University
    software validation.
  • Hekai Li Li, Southern Medical University. Zhujiang Hospital

    resources.

  • Jing Yan, Southern Medical University. Zhujiang Hospital. Zhujiang Hospital of Southern Medical University

    resources.

  • Man Long, Ultrasound Medical Center, Zhujiang Hospital of Southern Medical University, China

    resources

  • Xili Yang, Department of Cardiology of The First People’s Hospital of Foshan City, China
    formal analysis
  • Wei Wang, Department of Cardiology of The First People’s Hospital of Foshan City, China
    visualization
  • Weijie Li, Department of Cardiology of The First People’s Hospital of Foshan City, China
    data curation
  • Aihua Chen, Department of Health Management, Zhujiang Hospital, Southern Medical University, China

    Supervision

  • Yuanna Ling, Department of Nuclear Medicine of Zhujiang Hospital of Southern Medical University, China
    project administration writing-review & editing

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Published

2023-09-08

Issue

Vol. 59 (2023)

Section

Article

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How to Cite

Compound Danshen Dripping Pills pretreatment protects the heart from ischemia/reperfusion injury by enhancing autophagic flux . (2023). Brazilian Journal of Pharmaceutical Sciences, 59, e211035. https://doi.org/10.1590/s2175-97902023e211035

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