Effect of pancreatin on acute pancreatitis resulting from L-arginine administration in mice, a morphohistopathological and biochemical study
DOI:
https://doi.org/10.1590/s2175-97902023e21494Keywords:
Mice, Morphology, Biochemical parameters, Histopathology, Islands diameterAbstract
Acute pancreatitis (AP) is a life-unpleasant situation with contradictory and inadequate treatments. In this regard, the present study evaluated the effect of the possible pretreatment of lipase-pancreatin on L-arginine-induced AP. Forty adult mice were selected and divided into five groups: I) control group, II and III) AP groups (i.p.) receiving L-arginine of 2×300 and 2×400 mg/100 g body weight (b.w.), IV) AP (2×300 L-arginine) group + pancreatin (mice were i.p. injected by 350 U-lipase), and V) AP (2×400 L-arginine) group + pancreatin (mice were i.p. injected by 350 U-lipase). All AP groups displayed a significant increase in serum levels of ALT, AST, TBARS, and TNF-alpha compared to the control group. Moreover, pancreatic tissue edema, inflammation, and vacuolization of acinar cells were significantly higher in the untreated L-arginine group compared to the control and pancreatin groups. Conversely, the diameter of pancreatic islets significantly declined after induction of pancreatitis compared with control and pancreatin groups. Pancreatin treatment can be used in pancreatic dysfunction, however, this medicine showed no protective effect against L-arginine-induced AP in the mouse model.
Downloads
References
Abdel-Gawad SK. Therapeutic and protective effect of wheat germ oil on l-arginine induced acute pancreatitis in adult Albino rats. J Cell Sci Ther. 2015:1-7.
Abdelzaher WY, Ahmed SM, Welson NN, Marraiki N, Batiha GE-S, Kamel MY. Vinpocetine ameliorates L-arginine induced acute pancreatitis via Sirt1/Nrf2/TNF pathway and inhibition of oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020; 133: 110976.
Abu-Hilal M, McPhail M, Marchand L, Johnson CD. Malondialdehyde and superoxide dismutase as potential markers of severity in acute pancreatitis. JOP. 2006;7:185-192.
Affourtit C, Jastroch M, Brand MD. Uncoupling protein-2 attenuates glucose-stimulated insulin secretion in INS-1E insulinoma cells by lowering mitochondrial reactive oxygen species. Free Radic Biol Med. 2011;50:609-616.
Amiri A, Dehkordi RAF, Heidarnejad MS, Dehkordi MJ. Effect of the zinc oxide nanoparticles and thiamine for the management of diabetes in alloxan-induced mice: a stereological and biochemical study. Biol Trace Elem Res. 2018;181:258-264.
Aziz N, Kamel M, Rifaai R. Eff ects of hemin, a heme oxygenase-1 inducer in L-arginine-induced acute pancreatitis and associated lung injury in adult male albino rats. Endocr Regul. 2017; 51: 20-30.
Bhatia M, Brady M, Shokuhi S, Christmas S, Neoptolemos JP, Slavin J. Inflammatory mediators in acute pancreatitis. J Pathol. 2000;190:117-125.
Biczó G, Hegyi P, Dósa S, Shalbuyeva N, Berczi S, Sinervirta R, et al. The crucial role of early mitochondrial injury in L-lysine-induced acute pancreatitis. Antioxid Redox Signal. 2011;15:2669-2681.
Biradar S, Veeresh B. Screening of natural antioxidants by using L-arginine induced acute pancreatitis model. Int J Drug Dev Res. 2012;4:284-297.
Dawra R, Saluja AK. L-arginine-induced experimental acute pancreatitis. Pancreapedia. 2012;6:1-8.
Dawra R, Sharif R, Phillips P, Dudeja V, Dhaulakhandi D, Saluja AK. Development of a new mouse model of acute pancreatitis induced by administration of L-arginine. Am J Physiol Gastrointest Liver Physiol. 2007;292:G1009-G1018.
Dražić S, Sladoje N, Lindblad J. Estimation of Feret’s diameter from pixel coverage representation of a shape. Pattern Recognit Lett. 2016;80:37-45.
El-Ashmawy NE, Khedr NF, El-Bahrawy HA, Hamada OB. Anti-inflammatory and antioxidant effects of captopril compared to methylprednisolone in L-arginine-induced acute pancreatitis. Dig Dis Sci. 2018;63:1497-1505.
El Morsy EM, Ahmed MA, Carvedilol attenuates l-arginine induced acute pancreatitis in rats through modulation of oxidative stress and inflammatory mediators. Chem-Biol. Interact. 2020;327:109181.
Fatahian Dehkordi RA, Hamid M, Stereological estimation and morphological assessment of the endocrine pancreatic components in relation to sex in hen. Vet Res Forum. 2015;6:49-54.
Fusco R, Cordaro M, Siracusa R, D’Amico R, Genovese T, Gugliandolo E, et al. Biochemical evaluation of the antioxidant effects of hydroxytyrosol on pancreatitis-associated gut injury. Antioxidants. 2020;9:781.
Gukovsky I, Li N, Todoric J, Gukovskaya A, Karin M. Inflammation, autophagy, and obesity: common features in the pathogenesis of pancreatitis and pancreatic cancer. Gastroenterology. 2013;144:1199-1209. e1194.
Hegyi P, Pandol S, Venglovecz V, Rakonczay Z. The acinar-ductal tango in the pathogenesis of acute pancreatitis. Gut. 2011;60:544-552.
Hernández V, Miranda M, Pascual I, Sánchiz V, Almela P, Añón R, et al. Malondialdehyde in early phase of acute pancreatitis. Rev Esp Enferm Dig. 2011;103:563.
Hu F, Lou N, Jiao J, Guo F, Xiang H, Shang D. Macrophages in pancreatitis: Mechanisms and therapeutic potential. Biomed Pharmacother . 2020;131:110693.
Ianiro G, Pecere S, Giorgio V, Gasbarrini A, Cammarota G, Digestive enzyme supplementation in gastrointestinal diseases. Curr Drug Metab. 2016;17:187-193.
Johnson M, Hillier K. Pancreatin. 2011. In S. J. Enna and D. B. Bylund (eds), xPharm: The Comprehensive Pharmacology Reference. Elsevier Inc. p. 1-3
Kahl S, Schütte K, Glasbrenner B, Mayerle J, Simon P, Henniges F, et al. The effect of oral pancreatic enzyme supplementation on the course and outcome of acute pancreatitis: a randomized, double-blind parallel-group study. JOP. J Pancreas. 2014;15:165-174.
Kui B, Balla Z, Végh ET, Pallagi P, Venglovecz V, Iványi B, et al. Recent advances in the investigation of pancreatic inflammation induced by large doses of basic amino acids in rodents. Lab Invest. 2014;94:138-149.
Makhija R, Kingsnorth AN. Cytokine storm in acute pancreatitis. J Hepatobiliary Pancreat Surg. 2002;9:401-410.
Mayhew T. The new stereological methods for interpreting functional morphology from slices of cells and organs. Exp Physiol. 1991;76:639-665.
Mayhew TM. A review of recent advances in stereology for quantifying neural structure. J Neurocytol. 1992;21:313-328.
Mederos MA, Reber HA, Girgis MD. Acute Pancreatitis: A Review. JAMA. 2021;325:382-390.
Melo CM, Carvalho KMMB, de Sousa Neves JC, Morais TC, Rao VS, Santos FA, et al. α, β-amyrin, a natural triterpenoid ameliorates L-arginine-induced acute pancreatitis in rats. World J Gastroenterol. 2010;16:4272.
Miki A, Ricordi C, Sakuma Y, Yamamoto T, Misawa R, Mita A, et al. Divergent antioxidant capacity of human islet cell subsets: A potential cause of beta-cell vulnerability in diabetes and islet transplantation. PLoS One. 2018;13:e0196570.
Mirmalek SA, Gholamrezaei Boushehrinejad A, Yavari H, Kardeh B, Parsa Y, Salimi-Tabatabaee SA, et al. Antioxidant and anti-inflammatory effects of coenzyme Q10 on L-arginine-induced acute pancreatitis in rat. Oxid Med Cell Longev. 2016;2016:1-8.
Olatosin TM, Akinduko DS, Uche CZ. Antioxidant capacity of Moringa oleifera seed oil against CCl4-induced hepatocellular lipid peroxidation in wistar albino rats. J Exp Biol. 2014;4:514-518.
Pasari LP, Khurana A, Anchi P, Saifi MA, Annaldas S, Godugu C. Visnagin attenuates acute pancreatitis via Nrf2/ NFκB pathway and abrogates associated multiple organ dysfunction. Biomed Pharmacother . 2019;112:108629.
Pérez S, Pereda J, Sabater L, Sastre J. Redox signaling in acute pancreatitis. Redox biology. 2015;5:1-14.
Shrivastava P, Bhatia M. Essential role of monocytes and macrophages in the progression of acute pancreatitis. World J Gastroenterol . 2010;16:3995.
Sindhu RK, Ehdaie A, Farmand F, Dhaliwal KK, Nguyen T, Zhan C-D et al. Expression of catalase and glutathione peroxidase in renal insufficiency. Molecular Cell Research. 2005;1743:86-92.
Swentek L, Chung D, Ichii H. Antioxidant Therapy in Pancreatitis. Antioxidants. 2021;10:657.
Vaziri ND, Bai Y, Ni Z, Quiroz Y, Pandian R, Rodriguez-Iturbe B. Intra-renal angiotensin II/AT1 receptor, oxidative stress, inflammation, and progressive injury in renal mass reduction. J Pharmacol Exp Ther. 2007;323:85-93.
Wahba NS, Shaban SF, Kattaia AA, Kandeel SA. Efficacy of zinc oxide nanoparticles in attenuating pancreatic damage in a rat model of streptozotocin-induced diabetes. Ultrastruct Pathol. 2016;40:358-373.
Yenicerioglu A, Cetinkaya Z, Girgin M, Ustundag B, Ozercan IH, Ayten R, et al. Effects of trimetazidine in acute pancreatitis induced by L-arginine. Can J Surg. 2013;56:175.
Zhang X, Jin T, Shi N, Yao L, Yang X, Han C, et al. Mechanisms of pancreatic injury induced by basic amino acids differ between L-arginine, L-ornithine, and L-histidine. Front Physiol. 2019;9:1922.
Zhang Z, Wang Y, Dong M, Cui J, Rong D, Dong Q. Oxymatrine ameliorates L-arginine-induced acute pancreatitis in rats. Inflammation. 2012;35:605-613.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.
The on-line journal has open and free access.
