The Protective Effects of Geraniol Against Damage of Short Term Renal Ischemia-Reperfusion in Rats
DOI:
https://doi.org/10.1590/s2175-97902023e21248Keywords:
Free Radical , Antioxidant, Geraniol , Ischemia Reperfusion, KidneyAbstract
Ischemia/reperfusion (I/R) injury is one of the main causes of acute kidney injury. The pathological mechanisms underlying renal I/R injury are complex and remain uncertain. The protective effects of antioxidant properties of geraniol against renal ischemia reperfusion (I/R) damage were investigated in our study. 28 Wistar albino male rats were randomly selected and 4 groups of n = 7 were created. A right kidney nephrectomy surgery was conducted to all groups under anesthesia. 2 ml SF was given to Groups I and II, 50 mg/kg and 100 mg/ kg geraniol were administered intraperitoneally an hour before ischemia to Groups III and IV, respectively. Except for Group I, 45 minutes of ischemia and 4 hours of reperfusion were applied to the groups. At the end of the experiment, parameters related to oxidative stress and inflammation were determined by comparing kidney function, antioxidant enzyme activities and histological changes. Following comparison of BUN and CRE values with CAT and SOD values in tissue samples of Group I and Group II, an increase in Group II was observed and as a result I/R damage formation occurred. Values of geraniol-treated Group III and Group IV approximated to that of Group I, and that the 50 mg/kg geraniol dose proved more effective than 100 mg/kg geraniol.
Downloads
References
Akkoc A, Metin A, Kandirali E, Eroglu M, Yanik S, Ozcan F. The effects of prophylactic calcium dobesilate in experimental renal ischemia reperfusion injury. EUR Urol Suppl. 2010;9(6):577.
Al-Gubory KH, Fowler PA, Garrel C. The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes. Int J Biochem Cell Biol. 2010;42(10):1634-1650.
Amini N, Sarkaki A, Dianat M, Mard SA, Ahangarpour A, Badavi M. The renoprotective effects of naringin and trimetazidine on renal ischemia/reperfusion injury in rats through inhibition of apoptosis and downregulation of micoRNA-10a. Biomed Pharmacother. 2019;112:1-8.
Asgharpour M, Tolouian A, Bhaskar LVKS, Tolouian R, Massoudi N. Herbal antioxidants and renal ischemic-reperfusion injury; an updated review. J Nephropharmacol. 2021;10(1):e03.
Babakumar S, Vinothkumar V, Sankaranarayanan C, Srinivasan S. Geraniol, a natural monoterpene, ameliorates hyperglycemia by attenuating the key enzymes of carbohydrate metabolism in streptozotocin-induced diabetic rats. Pharm Biol. 2017;55(1):1442-1449.
Baykara B, Tekmen I, Pekcetin C, Ulukus C, Tuncel P, Sagol O, et al. The protective effect of carnosine and melatonin in ischemia-reperfusion injury in the rat liver. Acta histochemica. 2009;111(1):42-51.
Bayramoglu G, Kabay S, Ozden H, Ustuner MC, Uysal O, Bayramoglu A, et al. The effect of Echinacea on kidney and liver after experimental renalischemia reperfusion injury in the rats. Afr J Pharm Pharmacol. 2011;5(13):1561-1566.
Beauchamp C, Fridovic I. Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44(1):276-286.
Bhattamisra SK, Yan VLY, Lee CH, Kuean CH, Candasamy M, Liew YK, et al. Protective activity of geraniol against acetic acid and Helicobacter pylori-induced gastric ulcers in rats. J Tradit Complement Med. 2019;9(3):206-214.
Bullock JBJ, Wang BM. NMS Physiology 4th edition. Lippincott Williams Wilkins. Pennsylvania 2001; 289-296.
Can S, Canbek M. Protective Effects of Geraniol on Long Term Renal Ischemia/Reperfusion Injury in Rats. Braz Arch Biol Technol. 2019;62:e19180438.
Canbek M, Bayramoglu G, Senturk H, Oztopcu Vatan AP, Uyanoglu M, Ceyhan E, et al. The examination of protective effects of gallic acid against damage of oxidative stress during induced-experimental renal ischemia-reperfusion in experiment. Bratislava Med J. 2014;115(9):557-62.
Ceyhan E, Canbek M. Determining the Effects of Geraniol on Liver Regeneration Via the Nuclear Factor κB Pathway After Partial Hepatectomy. Altern Ther Health Med. 2017;23(3):38-45.
Chen W, Viljoen A. Geraniol A review of a commercially important fragrance material. S Afr J Bot. 2010;76(4):643-651.
Cho M, So I, Chun JN, Jeon JH. The antitumor effects of geraniol: Modulation of cancer hallmark pathways (Review). Int J Oncol. 2016;48(5):1772-1782.
Choi HS, Song HS, Ukeda H, Sawamura M. Radical-scavenging activities of citrus essential oils and their components: detection using 1,1-diphenyl-2-picrylhydrazyl. J Agric Food Chem. 2000;48(9):4156-4161.
Crespo R, Wei K, Rodenak-Kladniew B, Mercola M, Ruiz-Lozano P, Hurdato C. Effect of geraniol on rat cardiomyocytes and its potential use as a cardioprotective natural compound. Life sciences. 2017;172:8-12.
Elguindy NM, Yacout GA, Azab E. Amelioration of DENA-induced oxidative stress in rat kidney and brain by the essential oil of Elettaria cardamomum. BJBAS. 2018;7(3):299-305.
Gross GJ, Auchampach JA. Reperfusion injury: does it exist? J Mol Cell Cardiol. 2007;42(1):12-18.
Kalogeris T, Baines CP, Krenz M, Korthuis RC. Cell Biology of Ischemia/Reperfusion Injury. Int Rev Cell Mol Biol. 2012;298:229-317.
Kaya Y, Aral E, Coskun T, Erkasap N, Var A. Increased intraabdominal pressure impairs liver regeneration after partial hepatectomy in rats. J Surg Res. 2002;(108):250-257.
Kawamoto EM, Munhoz CD, Glezer I, Bahia VS, Caramelli P, Nitrini N, et al. Oxidative state in platelets and erythrocytes in aging and Alzheimer’s disease. Neurobiol Aging. 2005;26(6):857-64.
Kim SY, Lim JH, Park MR, Kim YJ, Park TI, Seo YW, et al. Enhanced Antioxidant Enzymes Are Associated with Reduced Hydrogen Peroxide in Barley Roots Under Saline Stress. J Biochem Mol Biol. 2005;38(2):218-24.
Korkmaz A, Kolankaya D. Protective Effect of Rutin on the Ischemia/Reperfusion Induced Damage in Rat Kidney. J Surg Res . 2010;164(2):309-15.
Lei Y, Fu P, Jun X, Cheng P. Pharmacological Properties of Geraniol - A Review. Planta Med. 2019;85(1):48-55.
Madankumar A, Jayakumar S, Devaki T. Geraniol, A Component of Plant Essential Oils Prevents Experimental Oral Carcinogenesis by Modulating Glycoprotein Abnormalities and Membrane Bound ATPASE’S. Int J Pharm Pharm Sci. 2013;5(1):416-420.
Mohammed MJ, Tadros MG, Michel HE. Geraniol protects against cyclophosphamide-induced hepatotoxicity in rats: Possible role of MAPK and PPAR-γ signaling pathways. Food Chem Toxicol. 2020;139:111251.
Onal A, Astarcıoglu H, Ormen M, Atila K, Sarioglu S. The beneficial effect of L-carnitine in rat renal ischemia-reperfusion injury. Turk J Trauma Emerg Surg. 2004;10(3):160-167.
Ozturk H, Cetinkaya A, Duzcu SE, Kin Tekce B, Ozturk H. Carvacrol attenuates histopathogic and functional impairments induced by bilateral renal ischemia/reperfusion in rats. Biomed Pharmacother . 2018;98:656-661.
Paller MS, Hoidal JR, Ferris TF. Oxygen free radicals in ischemic acute renal failure in the rat. J Clin Invest. 1984;74(4):1156.
Prasad SN. Mitigation of acrylamide-induced behavioral deficits, oxidative impairments and neurotoxicity by oral supplements of geraniol (a monoterpene) in a rat model. Chem Biol Interact. 2014;223:27-37.
Rekha KR, Sivakamasundari RI. Geraniol Protects Against the Protein and Oxidative Stress Induced by Rotenone in an In Vitro Model of Parkinson’s Disease. Neurochem Res. 2018;43(10):1947-1962.
Senturk, H, Yildiz, F. Protective effects of Olea europaea L.(Olive) leaf extract against oxidative stress injury generated with renal ischemia reperfusion. J Animal Plant Sci. 2018;28(4)1027-1033.
Sies H. Oxıdatıve stress: oxidants and antioxidants. Experimental Physiology. 1997;82(2):291-295.
Singh D, Chander V, Chopra K. Protective Effect of Catechin on Ischemia Reperfusion-Induced Renal Injury in Rats. Pharmacol Rep. 2005;57(1):70-76.
Theocharis SE, Margeli AP, Skaltsas SD, Spiliopoulou CA, Koutselinis AS. Induction of metallothionein in the liver of carbon tetrachloride intoxicated rats: an immunohistochemical study. Toxicology. 2001;161(1-2):129-138.
Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of chronic disease. Crit Rev Food Sci Nutr. 2004;44(4):275-295.
Williams P, Lopez H, Britt D, Chan C, Ezrin A, Hottendorf R. Characterization of renal ischemia-reperfusion injury in rats. J Pharmacol Toxicol Methods. 1997;37(1)1-7.
Woodbury W, Spencer AK, Stahman MA. An improved procedure using ferricyanide for detecting catalase isozymes. Anal Biochem . 1971;44(1):301-305.
Zimmerman B, Granger D. Reperfusion injury. Surg Clin North Am. 1992;72(1):65-83.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.
The on-line journal has open and free access.
