Cord factor producer Mycobacterium abscessus subsp. bolletii in asymptomatic immunocompetent host sputa samples

Authors

  • Beatriz Cardoso de Freitas Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Brazil
  • Jean Eduardo Meneguello Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Brazil https://orcid.org/0000-0003-1836-2950
  • Livia Fernandes Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Brazil
  • Rhayana Lemes Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Brazil
  • Regiane Bertin Lima Scodro Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Brazil
  • Vera Lucia Dias Siqueira Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Brazil
  • Katiany Rizzieri Caleffi-Ferracioli Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Brazil https://orcid.org/0000-0003-0345-1600
  • Rosilene Fressatti Cardoso Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Brazil https://orcid.org/0000-0001-6228-4780

DOI:

https://doi.org/10.1590/s2175-97902022e19504

Keywords:

Nontuberculous mycobacteria, Tuberculosis, Diagnosis, Microscopy, Molecular diagnosis

Abstract

We report a case of Mycobacterium abscessus subsp. bolletii colonization in upper respiratory tract of an immunocompetent patient, who was misdiagnosed as tuberculosis by Acid Fast Bacilli (AFB) and cord factor formation observed directly from the sputa culture in liquid medium. This fact reflected a significant impact on the individual case’s life and showed the importance to identify the mycobacteria isolated from clinical sample at species level, and to determine the true implication of nontuberculous mycobacteria (NTM) detected in clinical samples.

Downloads

References

Brasil, Ministério da Saúde. Manual de recomendações para o controle da tuberculose no Brasil. 1a edição. Ministério da Saúde. Brasília. 2011. Available at: http://bvsms.saude.gov.br/bvs/publicacoes/manual_recomendacoes_controle_tuberculose_brasil.pdf

» http://bvsms.saude.gov.br/bvs/publicacoes/manual_recomendacoes_controle_tuberculose_brasil.pdf

Carter YSMC, Ratkiewicz IN. Cord formation in BACTEC medium is a reliable, rapid method for presumptive identification of Mycobacterium tuberculosis complex. J Clin Microbiol. 1998;36(9):2769-71.

Harland CW, Rabuka D, Bertozzi CR, Parthasarathy R. The Mycobacterium tuberculosis virulence factor trehalose dimycolate imparts desiccation resistance to model mycobacterial membranes. Biophys J. 2008;94(12):4718-24. doi:10.1529/biophysj.107.125542.

» https://doi.org/10.1529/biophysj.107.125542

Howard ST, Rhoades E, Recht J, Pang X, Alsup A, Kolter R, et al. Spontaneous reversion of Mycobacterium abscessus from a smooth to a rough morphotype is associated with reduced expression of glycopeptidolipid and reacquisition of an invasive phenotype. Microbiology. 2006;152(Pt. 6):1581- 90. doi:10.1099/mic.0.28625-0.

» https://doi.org/10.1099/mic.0.28625-0

Li G, Pang H, Guo Q, Huang M, Tan Y, Li C, Wei J, Xia Y, Jiang Y, Zhao X, Liu H, Zhao LL, Liu Z, Xu D, Wan K. Antimicrobial susceptibility and MIC distribution of 41 drugs against clinical isolates from China and references strains of nontuberculous mycobacteria. Int J Antimicrob Agents. 2017;49(3):364-374.doi: 10.1016/j.ijantimicag.2016.10.024.

» https://doi.org/10.1016/j.ijantimicag.2016.10.024

Julián E, Roldán M, Sánchez-Chardi A, Astola O, Agustí G, Luquin M. Microscopic cords, a virulence-related characteristic of Mycobacterium tuberculosis, are also present in nonpathogenic mycobacteria. J Bacteriol. 2010;192(7):1751-60. doi:10.1128/JB.01485-09.

» https://doi.org/10.1128/JB.01485-09

Kim Byoung-Jun, Kim B-R, Lee S-Y, Kook Y-H, Kim Bum-Joon. Rough colony morphology of Mycobacterium massiliense Type II genotype is due to the deletion of glycopeptidolipid locus within its genome. BMC Genomics. 2013;14:890. doi:10.1186/1471-2164-14-890.

» https://doi.org/10.1186/1471-2164-14-890

Medjahed H, Reyrat J-M. Construction of Mycobacterium abscessus defined glycopeptidolipid mutants: comparison of genetic tools. Appl Environ Microbiol. 2009;75(5):1331-8. doi:10.1128/AEM.01914-08.

» https://doi.org/10.1128/AEM.01914-08

Middlebrook BYG, Dubos RENJ, Pierce C. Virulence and morphological characteristics of mammalian tubercle bacilli. J Exp Med. 1947;86(2):175-184. doi: 10.1084/jem.86.2.175

» https://doi.org/10.1084/jem.86.2.175

Pang H, Li G, Wan L, Jiang Y, Liu H, Zhao X, et al. In vitro drug susceptibility of 40 international reference rapidly growing mycobacteria to 20 antimicrobial agents. Int J Clin Exp Med. 2015;8(9):15423-31.

Rüger K, Hampel A, Billig S, Rücker N, Suerbaum S, Bange FC. Characterization of rough and smooth morphotypes of Mycobacterium abscessus isolates from clinical specimens. J Clin Microbiol . 2014;52(1):244-50. doi:10.1128/JCM.01249-13.

» https://doi.org/10.1128/JCM.01249-13

Sanguinetti M, Ardito F, Fiscarelli E, Sorda MLA, Argenio PD, Ricciotti G, et al. Fatal pulmonary infection due to multidrug-resistant Mycobacterium abscessus in a patient with cystic fibrosis. J Clin Microbiol . 2001;39(2):816-9. doi: 10.1128/JCM.39.2.816-819.2001

» https://doi.org/10.1128/JCM.39.2.816-819.2001

Van Ingen J, Kuijper EJ. Drug susceptibility testing of nontuberculous mycobacteria. Future Microbiol. 2014;9(9):1095-110.doi: 10.2217/fmb.14.60.

» https://doi.org/10.2217/fmb.14.60

Yamagami H, Matsumoto T, Fujiwara N, Arakawa T, Kaneda K, Yano I, et al.Trehalose 6,6′-dimycolate (cord factor) of Mycobacterium tuberculosis induces foreign-body- and hypersensitivity-type granulomas in mice. Infect Immun. 2001;69(2):810-5. doi:10.1128/IAI.69.2.810-815.2001.

» https://doi.org/10.1128/IAI.69.2.810-815.2001

World Health Organization. World health statistics 2016: monitoring health for the SDGs, sustainable development goals. World Health Organization. 2018.

Downloads

Published

2022-11-23

Issue

Vol. 58 (2022)

Section

Original Article

License

Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

The on-line journal has open and free access.

How to Cite

Cord factor producer Mycobacterium abscessus subsp. bolletii in asymptomatic immunocompetent host sputa samples. (2022). Brazilian Journal of Pharmaceutical Sciences, 58. https://doi.org/10.1590/s2175-97902022e19504