Simultaneous determination of paracetamol, dextromethorphan, chlorpheniramine, pseudoephedrine and major impurities of paracetamol and pseudoephedrine by using capillary electrophoresis

Authors

  • Beytul Yener Gazi University, Faculty of Pharmacy, Department of Analytical Chemistry, 06330, Ankara, Turkey
  • Cem Erkmen Ankara University https://orcid.org/0000-0001-5944-3912
  • Bengi Uslu Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560, Ankara, Turkey
  • Nilgun Gunden Goger Gazi University, Faculty of Pharmacy, Department of Analytical Chemistry, 06330, Ankara, Turkey

DOI:

https://doi.org/10.1590/s2175-97902022e19836%20%20

Keywords:

Capillary electrophoresis, Chlorpheniramine, Dextromethorphan, Paracetamol, Pseudoephedrine

Abstract

A capillary electrophoresis method was developed for the first time and optimized for the
determination of paracetamol, pseudoephedrine, dextromethorphan, chlorpheniramine,
4-aminophenol and ephedrine in tablet formulation. Optimum electrophoretic conditions were
achieved by using a background electrolyte of 75 mmol L-1 sodium borate buffer at pH 8.0, a
capillary temperature of 30°C, a separation voltage of 30 kV and a pressure injection of the sample
at 50 mbar for 10 s. Calibration graphs showed a good linearity with a coefficient of determination
(R2
) of at least 0.999 for all compounds. Intraday and interday precision (expressed as relative
standard deviation (RSD) %) were lower than 1.39% for capillary electrophoresis method. The
developed method was demonstrated to be simple and rapid for the determination of paracetamol,
pseudoephedrine, dextromethorphan, chlorpheniramine, 4-aminophenol and ephedrine in tablet
formulation providing recoveries in the range between 99.62 and 100.57% for all analytes.

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Published

2022-11-23

Issue

Vol. 58 (2022)

Section

Original Article

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How to Cite

Simultaneous determination of paracetamol, dextromethorphan, chlorpheniramine, pseudoephedrine and major impurities of paracetamol and pseudoephedrine by using capillary electrophoresis. (2022). Brazilian Journal of Pharmaceutical Sciences, 58. https://doi.org/10.1590/s2175-97902022e19836

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