Anti-diabetic effect of betulinic acid on streptozotocinnicotinamide induced diabetic male mouse model

Authors

  • Golshan Arzani Birgani Ahvaz Jundishapur University of Medical Science. Department of Physiology, Student Research Committee
  • Akram Ahangarpour Ahvaz Jundishapur University of Medical Sciences. School of Medicine. Physiology Research Center. Department of Physiology
  • Layasadat Khorsandi Ahvaz Jundishapur University of Medical Sciences. Cell & Molecular Research Center. School of Medicine. Department of Anatomical Sciences
  • Hadi Fathi Moghaddam Ahvaz Jundishapur University of Medical Sciences. School of Medicine. Physiology Research Center. Department of Physiology

DOI:

https://doi.org/10.1590/s2175-97902018000217171

Keywords:

Betulinic acid. Diabetes, Mouse, Streptozotocin-nicotinamide

Abstract

Diabetes is a metabolic disease caused by abnormal insulin secretion or action. In the present study, the effects of betulinic acid (BA, a triterpene) are evaluated on glucose, α-amylase and plasma insulin levels, insulin resistance and the histopathology of pancreatic islets in streptozotocin-nicotinamide (STZNA) diabetic mice. Seventy adult male NMRI mice were randomly divided into seven groups: control, sham, diabetic, diabetic treated with BA (10, 20 and 40 mg/kg) and diabetic treated with metformin (200 mg/kg). Diabetes was induced in mice by intraperitoneal injection of streptozotocin 50 mg/kg after a dose of nicotinamide 120 mg/kg. Two weeks after treatment with BA, blood samples were collected for measuring glucose, α-amylase and insulin levels, and the pancreas was isolated for histopathology evaluation. Diabetes reduced the number and diameter of pancreatic islets, and increased α-amylase and insulin resistance. BA treatment reduced blood glucose, α-amylase and improved insulin sensitivity as well as pancreas histopathology. In addition, BA showed stronger effects on the pancreatic histology and insulin resistance compared to the metformin group.

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Published

2018-07-26

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Articles

How to Cite

Anti-diabetic effect of betulinic acid on streptozotocinnicotinamide induced diabetic male mouse model. (2018). Brazilian Journal of Pharmaceutical Sciences, 54(2), e17171. https://doi.org/10.1590/s2175-97902018000217171