Development and validation of a fluorimetric method to determine curcumin in lipid and polymeric nanocapsule suspensions

Authors

  • Letícia Mazzarino Federal University of Santa Catarina; Department of Pharmaceutical Sciences; Laboratory of Pharmaceutical Technology
  • Ismael Casagrande Bellettini Federal University of Santa Catarina; Department of Chemistry; Laboratory of Polymer and Surfactant Solutions
  • Edson Minatti Federal University of Santa Catarina; Department of Chemistry; Laboratory of Polymer and Surfactant Solutions
  • Elenara Lemos-Senna Federal University of Santa Catarina; Department of Pharmaceutical Sciences; Laboratory of Pharmaceutical Technology

DOI:

https://doi.org/10.1590/S1984-82502010000200008

Keywords:

Curcumin^i1^sdeterminat, Fluorimetric method^i1^squantitative analy, Polymeric nanocapsules^i1^sevaluat, Lipid nanocapsules^i1^sevaluat

Abstract

A simple, rapid, and sensitive fluorimetric method was developed and validated to quantify curcumin in lipid and polymeric nanocapsule suspensions, using acetonitrile as a solvent. The excitation and emission wavelengths were set at 397 nm and 508 nm, respectively. The calibration graph was linear from 0.1 to 0.6 µg/mL with a correlation coefficient of 0.9982. The detection and quantitation limits were 0.03 and 0.10 µg/mL, respectively. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of curcumin content in lipid and polymeric nanocapsule suspensions during the early stage of formulation development.

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Published

2010-06-01

Issue

Vol. 46 No. 2 (2010)

Section

Original Papers

License

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

The on-line journal has open and free access.

How to Cite

Development and validation of a fluorimetric method to determine curcumin in lipid and polymeric nanocapsule suspensions . (2010). Brazilian Journal of Pharmaceutical Sciences, 46(2), 219-226. https://doi.org/10.1590/S1984-82502010000200008